胸骨正中切口与左侧肋间切口开胸制备心肌梗死动物模型的比较

发布时间：2018-09-19 20:40

【摘要】：背景:许多治疗冠心病的研究依赖于心肌梗死动物模型的建立。探索创伤小、易操作、安全、存活时间长、存活率高的心肌梗死动物模型制作方法具有重要意义。目的:比较两种开胸结扎冠状动脉制作心肌梗死动物模型方法的优劣。方法:健康雄性新西兰大耳白兔30只,随机分为3组,每组10只。胸骨正中切口组、胸骨左缘切口组开胸后结扎冠状动脉前降支,假手术组不结扎冠状动脉。监测手术时间、术中出血量、术后进食量、进食恢复时间。术后24 h内测心肌酶学指标,术后4周检测超声心动图。结果与结论:胸骨正中切口组及胸骨旁切口组结扎冠状动脉后心电图检查均出现不同程度的ST段弓背样抬高。胸骨正中切口组造模成功率为70%、胸骨左缘切口组造模成功率为80%。两组术后24 h内心肌酶学指标升高,与术前相比差异有显著性意义(P0.05);术后4周左室射血分数、左室短轴缩短率降低,与术前相比差异有显著性意义(P0.05)。与胸骨左缘切口组比较,胸骨正中切口组造模成功所需的手术时间少,术中出血量少,术后恢复进食时间短,术后进食量较多,差异均有显著性意义(P0.05),结果表明采用胸骨正中开胸法结扎冠状动脉更适合用于制作心肌梗死动物模型。
[Abstract]:Background: many studies on the treatment of coronary heart disease depend on the establishment of animal models of myocardial infarction. It is of great significance to explore an animal model of myocardial infarction with small trauma, easy operation, safety, long survival time and high survival rate. Objective: to compare the advantages and disadvantages of two methods of coronary artery ligation for myocardial infarction. Methods: 30 healthy male New Zealand white rabbits were randomly divided into 3 groups with 10 rabbits in each group. In the median sternal incision group, the left sternal margin incision group ligated the anterior descending coronary artery after thoracotomy, but the sham operation group did not ligate the coronary artery. Monitor the time of operation, blood loss during operation, food intake and recovery time after operation. Myocardial enzymes were measured 24 hours after operation and echocardiography 4 weeks after operation. Results and conclusion: after ligation of coronary artery in the median sternum incision group and parasternal incision group electrocardiogram examination showed different degree of ST segment dorsal elevation. The success rate of model making was 70 in the median sternal incision group and 80 in the left sternal margin incision group. The myocardial enzymatic indexes in the two groups increased within 24 hours after operation (P0.05), the left ventricular ejection fraction (LVEF) and the shortening rate of left ventricular short axis decreased at 4 weeks after operation, and there was a significant difference between the two groups (P0.05). Compared with the left sternum incision group, the median sternal incision group needed less operative time, less intraoperative bleeding, shorter recovery time and more postoperative food intake. The difference was significant (P0.05). The results showed that the central sternotomy ligation of coronary artery was more suitable for the establishment of myocardial infarction animal model.
【作者单位】：上海市第六人民医院南院;上海市奉贤区中心医院超声医学科;上海市松江区中心医院ICU;上海市松江区中心医院胸外科;
【基金】：上海市奉贤区科委资助项目(20111003)~~
【分类号】：R-332;R542.22

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