中国不同疫区L.d.ITS序列分析及L.p.PAL、HSP60基因克隆和真核表达
发布时间：2018-10-08 18:48
【摘要】：利什曼病是WHO/TDR列出的对人类危害严重的六类热带病之一。利什曼原虫属包含很多不同的种和亚种,其分类相当复杂。在世界范围内,利什曼原虫不同的种及亚种可引起不同类型的利什曼病,它们具有不同的流行病学特征。利什曼原虫种株的分析和鉴定对于提高临床疗效、控制此病的流行有重要意义。在我国,主要流行的是由杜氏利什曼原虫(Leishmania donovani,L.d.)导致的内脏利什曼病,即黑热病。该病对人体危害严重,是威胁患者生命的人兽共患病。我国的内脏利什曼病流行区根据地理分布和传染源不同分为荒漠型(野生动物源型)、山丘型(犬源型)和平原型(人源型),不同疫区的内脏利什曼病在患者发病年龄、治疗效果、是否易复发和有无保虫宿主等方面有着明显的差异,但病原体形态难以区别。传统的种株分析方法主要依据地理位置、临床表现、流行病学特征、原虫形态等外在特征进行分类,这对确定我国不同疫区利什曼原虫种株之间的关系往往不够。在现代分类研究中,分子生物学技术的运用越来越多。多年以来,为从分子水平揭示我国不同疫区利什曼原虫种株间的差异,从而为黑热病的防治提供科学依据,在该领域进行了许多极有意义的研究,如对利什曼原虫kDNA、nDNA的研究,用kDNA PCR-SSCP、RAPD分析等多种方法对我国不同疫区利什曼原虫分离株进行它们之间同源性的分析及种株鉴定。但由于其复杂性,出现了一些不一致的结论。因此我们有必要进一步
[Abstract]:Leishmaniasis is one of the six serious tropical diseases listed by WHO/TDR. Leishmania consists of many different species and subspecies, and its taxonomy is quite complex. In the world, different species and subspecies of Leishmania spp can cause different types of leishmaniasis, and they have different epidemiological characteristics. The analysis and identification of Leishmania protozoa strains is of great significance in improving the clinical efficacy and controlling the prevalence of the disease. In China, the main epidemic is by the Leishmania du Leishmania protozoa (Leishmania donovani,L.d.) The resulting visceral leishmaniasis is leishmaniasis, or leishmaniasis. The disease is a serious threat to the human body, is a life-threatening zoonosis. Visceral leishmaniasis (visceral leishmaniasis) is divided into desert type (wild animal type), hilly type (canine type) and archetype (human type) according to geographical distribution and source of infection in China. Visceral leishmaniasis (visceral leishmaniasis) in different epidemic areas is at the onset age of the patients. There were significant differences in the therapeutic effect, recurrence and host, but it was difficult to distinguish the morphology of pathogens. The traditional species and plant analysis methods are mainly classified according to geographical location, clinical manifestations, epidemiological characteristics, protozoa morphology and other external characteristics, which is often insufficient to determine the relationship between Leishmania species strains in different epidemic areas of China. In the modern taxonomy research, molecular biology technology is applied more and more. In order to reveal the differences of Leishmania protozoa species in different epidemic areas of China at molecular level and to provide scientific basis for the control of leishmaniasis, many meaningful studies have been carried out in this field, such as the study of Leishmania protozoa kDNA,nDNA. The homology and species identification of Leishmania spp isolates in different epidemic areas of China were analyzed by kDNA PCR-SSCP,RAPD analysis. However, due to its complexity, there are some inconsistent conclusions. So we need to go further.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R383
本文编号：2257878
[Abstract]:Leishmaniasis is one of the six serious tropical diseases listed by WHO/TDR. Leishmania consists of many different species and subspecies, and its taxonomy is quite complex. In the world, different species and subspecies of Leishmania spp can cause different types of leishmaniasis, and they have different epidemiological characteristics. The analysis and identification of Leishmania protozoa strains is of great significance in improving the clinical efficacy and controlling the prevalence of the disease. In China, the main epidemic is by the Leishmania du Leishmania protozoa (Leishmania donovani,L.d.) The resulting visceral leishmaniasis is leishmaniasis, or leishmaniasis. The disease is a serious threat to the human body, is a life-threatening zoonosis. Visceral leishmaniasis (visceral leishmaniasis) is divided into desert type (wild animal type), hilly type (canine type) and archetype (human type) according to geographical distribution and source of infection in China. Visceral leishmaniasis (visceral leishmaniasis) in different epidemic areas is at the onset age of the patients. There were significant differences in the therapeutic effect, recurrence and host, but it was difficult to distinguish the morphology of pathogens. The traditional species and plant analysis methods are mainly classified according to geographical location, clinical manifestations, epidemiological characteristics, protozoa morphology and other external characteristics, which is often insufficient to determine the relationship between Leishmania species strains in different epidemic areas of China. In the modern taxonomy research, molecular biology technology is applied more and more. In order to reveal the differences of Leishmania protozoa species in different epidemic areas of China at molecular level and to provide scientific basis for the control of leishmaniasis, many meaningful studies have been carried out in this field, such as the study of Leishmania protozoa kDNA,nDNA. The homology and species identification of Leishmania spp isolates in different epidemic areas of China were analyzed by kDNA PCR-SSCP,RAPD analysis. However, due to its complexity, there are some inconsistent conclusions. So we need to go further.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R383
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