面向微流控芯片的微量血清分离方法的研究
发布时间：2018-10-09 15:49
【摘要】： 为了满足微全分析系统对样品分离的集成度要求,研究一种新型的微量血清分离技术已成为一个急待解决的问题。论文在查阅大量国内外相关资料的基础上提出采用微过滤法和离心分离的基本方案,并结合血清分离的特殊性,对微血清分离器进行了理论分析、器件制作及实验研究。 在研究微过滤理论的基础上,利用MEMS技术设计制作了一种条栅状的硅微结构的过滤器,并对其进行了实验研究。结果表明,该过滤器能有效去除悬液中直径14μm以上的微粒,但对具有较强变形能力的红细胞过滤不彻底。 对毛细管离心分离进行了原理研究和实验分析。在此基础上设计并制作了两种不同封装形式的离心式血清微分离芯片——全PDMS血清微分离芯片和硅-PDMS混合式血清微分离芯片,实现了微升量级血清的分离。文中分别介绍了两种芯片的制作工艺,并对其性能进行了实验分析。分析讨论了材料表面性质、微通道管径和离心运行参数对血清分离结果的影响。分析讨论了硅模表面粗糙度和PDMS固化温度对芯片键合强度的影响。 为了解决血清分离后的取样问题,在离心式分离芯片的基础上,将微阀和气泵进行了集成设计。利用改变表面张力可控制流体在微通道的流动的原理,设计了实现血液自动进样的毛细微泵;通过改变微通道几何尺寸的实验,设计了可使流体在通道狭窄处止流的毛细微阀。阀的操作模式表明,此类被动阀适合于在低压、一次性使用的场合。
[Abstract]:In order to meet the requirement of integration of microanalysis system for sample separation, it has become an urgent problem to study a new microserum separation technology. On the basis of consulting a large number of related data at home and abroad, this paper puts forward the basic scheme of microfiltration and centrifugal separation, and combines the particularity of serum separation, carries on the theoretical analysis, the device fabrication and the experimental research to the microserum separator. On the basis of studying the theory of microfiltration, a kind of silicon microstructure filter with strip gate is designed and fabricated by using MEMS technology, and its experimental study is carried out. The results show that the filter can effectively remove the particles over 14 渭 m in diameter in the suspension, but it is not complete for the red blood cells with strong deformability. The principle and experimental analysis of capillary centrifugation were studied. On the basis of this, two kinds of centrifugal serum microseparation chips, full PDMS serum microseparation chip and silicon-PDMS mixed serum microseparation chip were designed and fabricated, which realized the separation of microserum in the order of magnitude. In this paper, the fabrication process of two kinds of chips are introduced, and their performances are analyzed experimentally. The effects of material surface properties, microchannel diameter and centrifugal operation parameters on serum separation results were analyzed and discussed. The effects of surface roughness and PDMS curing temperature on bonding strength of silicon die were analyzed and discussed. In order to solve the problem of sampling after serum separation, the microvalve and air pump were designed on the basis of centrifugal separation chip. Based on the principle that the fluid can be controlled by changing the surface tension in the microchannel, a capillary pump is designed to realize the automatic injection of blood, and by changing the geometric dimension of the microchannel, the capillary fine valve is designed to stop the fluid from flowing at the narrow point of the channel. The operating mode of the valve indicates that this type of passive valve is suitable for low pressure, one-time use.
【学位授予单位】：中国科学院研究生院（长春光学精密机械与物理研究所）
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-331
本文编号：2259893
[Abstract]:In order to meet the requirement of integration of microanalysis system for sample separation, it has become an urgent problem to study a new microserum separation technology. On the basis of consulting a large number of related data at home and abroad, this paper puts forward the basic scheme of microfiltration and centrifugal separation, and combines the particularity of serum separation, carries on the theoretical analysis, the device fabrication and the experimental research to the microserum separator. On the basis of studying the theory of microfiltration, a kind of silicon microstructure filter with strip gate is designed and fabricated by using MEMS technology, and its experimental study is carried out. The results show that the filter can effectively remove the particles over 14 渭 m in diameter in the suspension, but it is not complete for the red blood cells with strong deformability. The principle and experimental analysis of capillary centrifugation were studied. On the basis of this, two kinds of centrifugal serum microseparation chips, full PDMS serum microseparation chip and silicon-PDMS mixed serum microseparation chip were designed and fabricated, which realized the separation of microserum in the order of magnitude. In this paper, the fabrication process of two kinds of chips are introduced, and their performances are analyzed experimentally. The effects of material surface properties, microchannel diameter and centrifugal operation parameters on serum separation results were analyzed and discussed. The effects of surface roughness and PDMS curing temperature on bonding strength of silicon die were analyzed and discussed. In order to solve the problem of sampling after serum separation, the microvalve and air pump were designed on the basis of centrifugal separation chip. Based on the principle that the fluid can be controlled by changing the surface tension in the microchannel, a capillary pump is designed to realize the automatic injection of blood, and by changing the geometric dimension of the microchannel, the capillary fine valve is designed to stop the fluid from flowing at the narrow point of the channel. The operating mode of the valve indicates that this type of passive valve is suitable for low pressure, one-time use.
【学位授予单位】：中国科学院研究生院（长春光学精密机械与物理研究所）
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-331
【引证文献】
相关博士学位论文 前1条
1 渠柏艳;基于血液样品和微流控芯片技术的细胞分离、PCR扩增及DNA测序方法研究[D];东北大学;2009年
，本文编号：2259893
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