抗体序列结构分析系统的建立与应用

发布时间：2018-10-10 07:27

【摘要】：随着生物技术及信息技术的蓬勃发展,免疫学的研究方式在后基因组时代也将发生根本性的变革。我们不仅要面对已经存在的海量数据,还要关注那些成指数增长的新的免疫学相关数据。因此如何提高对这些信息的提取和分析能力是进行有效的免疫学研究的重要保证。目的:本研究项目旨在利用现有抗体相关生物信息资源,开发具有自主知识产权的软件工具,建立一个功能完备的,适用于抗体及其基因序列分析的平台。同时利用该系统对现有的治疗性抗体进行有效分析,为其进一步的抗体药物改造提供可靠的理论依据。从而试图改变传统的实验室研究模式,提高研究效率,促进抗体的研究以及相关药物的应用开发。方法:设计了性能良好,风格统一,用户友好的生物应用接口和用户界面;整合了已有的生物信息应用工具、处理程序,建立了功能完善,文档和支持强大的生物应用系统平台;同时针对抗体研究的特殊性,开发了具有特殊处理功能的程序,并将研究密切相关的数据和信息资源本地化以方便应用开发.而后利用该系统对抗-gp120抗体和鼠HAb18单克隆抗体进行了相关分析。结果:1.抗体序列分析的系统的搭建:成功开发了一套具有自主知识产权的用于抗体序列分析的生物信息软件集成系统AbSAS,该系统能成功实现对抗体核酸及蛋白质一级序列的分析,并可利用Threading及同源模建的方法实现对抗体三维结构的模拟,是一较为全面的抗体分析平台。2.现有抗体分
[Abstract]:With the rapid development of biotechnology and information technology, the research methods of immunology will also undergo fundamental changes in the post-genome era. Not only do we have to deal with the huge amounts of data that already exist, but we also have to look at new immunological data that are exponentially growing. Therefore, how to improve the ability of extracting and analyzing these information is an important guarantee for effective immunological research. Objective: the purpose of this research project is to develop a software tool with independent intellectual property rights using existing antibody related biological information resources and to establish a functional platform suitable for antibody and gene sequence analysis. At the same time, the system is used to analyze the existing therapeutic antibodies effectively, which provides a reliable theoretical basis for the further modification of antibody drugs. Therefore, we try to change the traditional laboratory research model, improve the efficiency of research, promote the research of antibodies and the application and development of related drugs. Methods: the bioapplication interface and user interface with good performance, uniform style and user friendly were designed, and the existing bioinformatics application tools and procedures were integrated, and the functions were improved. At the same time, according to the particularity of antibody research, a program with special processing function is developed, and the closely related data and information resources are localized to facilitate application and development. Then the system was used to analyze the correlation between anti-gp 120 antibody and mouse HAb18 monoclonal antibody. The result is 1: 1. The establishment of antibody sequence analysis system: a set of bioinformatics software integrated system (AbSAS,) with independent intellectual property rights for antibody sequence analysis has been successfully developed. The system can successfully realize the analysis of antibody nucleic acid and protein first order sequence. The method of Threading and homologous modeling can be used to simulate the three-dimensional structure of antibody, which is a more comprehensive antibody analysis platform. 2. Existing antibody score
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：S852.43;R392.1

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