硝基苯对于大白鼠心脏和肝脏的危害以及维生素C的对抗作用研究

发布时间：2018-10-11 06:36

【摘要】： 目的:观察硝基苯对于大白鼠的血液、心脏和肝脏的影响,探讨维生素C对于硝基苯危害的对抗作用。实验方法:以大白鼠为实验对象,测量心肌力学指标(左心室收缩压、左心室舒张末期压、左心室最大收缩速率和左心室最大舒张速率)、血液(平均动脉压、红细胞数)和组织生化(心脏的SOD、MDA,肝脏的SOD、MDA)的变化。实验结果:1.与对照组和溶剂组相比,硝基苯对于大白鼠的心肌力学指标明显呈剂量依赖性影响,左心室最大舒张速率在15mg/kg硝基苯组发生了显著的变化(与对照组相比下降了40.53%),其余的指标均在45mg/kg硝基苯组有显著变化(与对照组比较,左心室收缩压、左心室舒张末期压左心室最大收缩速率分别变化了-27.48%、+77.94%、-63.78%)。随着硝基苯剂量的增加,心肌力学的各个指标变化越来越大。2.与对照组和溶剂组相比,红细胞数的变化尤其显著。在15mg/kg组,红细胞数的数量即呈现明显的下降(与对照组相比,下降了8.39%)。随着硝基苯剂量的增加,红细胞数的下降越来越大,呈一种剂量依赖性。动脉平均压在45mg/kg硝基苯组呈现出明显的下降(与对照组相比,下降38.5%),此后随着硝基苯剂量的增加,动脉平均压越来越低。3.与对照组和溶剂组相比,心脏和肝脏的MDA在15mg/kg即呈现出明显的变化(与对照组相比,MDA分别增加74.74%和125.03%)。随着硝基苯剂量的增加,MDA则越来越高。心脏和肝脏的SOD在15mg/kg时稍有升高,但是不显著,在45mg/kg硝基苯组SOD开始出现明显下降(与对照组相比,心脏和肝脏的SOD分别下降49.54%和67.45%),随着硝基苯量的增加,SOD越来越低。4.维生素C对抗硝基苯组,除了心脏的SOD有明显的升高外(与45mg/kg硝基苯组相比,45mg/kgNB+0.8g/kgVC组升高了11.56%),其他的各项指标都没有明显的变化。结论:硝基苯对于心肌力学指标(左心室收缩压、左心室舒张末期压、左心室最大收缩速率和左心室最大舒张速率)、红细胞数、心脏和肝脏的SOD、MDA均呈剂量依赖性的影响。随着硝基苯剂量的增加,左心室舒张末期压、心脏和肝脏的MDA逐渐升高,其他指标则逐渐降低。维生素C对于硝基苯引起的危害作用无明显的对抗。
[Abstract]:Aim: to observe the effects of nitrobenzene on blood, heart and liver of rats and to explore the antagonistic effect of vitamin C on the damage of nitrobenzene. Methods: myocardial mechanical indexes (left ventricular systolic pressure, left ventricular end-diastolic pressure, left ventricular maximum systolic velocity and left ventricular maximal diastolic velocity), blood (mean arterial pressure) were measured in rats. Changes in erythrocyte count and tissue biochemistry (SOD,MDA of the heart's SOD,MDA, liver). Experimental results: 1. Compared with the control group and the solvent group, nitrobenzene had a dose-dependent effect on myocardial mechanical indexes of rats. The maximal left ventricular diastolic velocity changed significantly in the 15mg/kg nitrobenzene group (decreased by 40.53% compared with the control group), and the other indexes were significantly changed in the 45mg/kg nitrobenzene group (compared with the control group, left ventricular systolic pressure, left ventricular end-diastolic pressure? The maximal systolic rate of left ventricle changed -27.48% and 77.94% -63.78% respectively. With the increase of nitrobenzene dose, the indexes of myocardial mechanics change more and more. 2. 2. Compared with control group and solvent group, the change of erythrocyte number was especially significant. In the 15mg/kg group, the number of erythrocytes decreased significantly (8.39% compared with the control group). With the increase of nitrobenzene dose, the number of erythrocytes decreased more and more in a dose-dependent manner. The mean arterial pressure decreased significantly in 45mg/kg nitrobenzene group (38.5% compared with the control group), and then decreased with the increase of nitrobenzene dose. Compared with the control group and the solvent group, the MDA of heart and liver showed obvious changes in 15mg/kg (MDA increased 74.74% and 125.03% respectively compared with the control group). With the increase of nitrobenzene dose, MDA is higher and higher. The SOD of heart and liver increased slightly at 15mg/kg, but not significantly. In 45mg/kg nitrobenzene group, SOD began to decrease significantly (compared with control group, the SOD of heart and liver decreased by 49.54% and 67.45%, respectively). With the increase of nitrobenzene content, SOD decreased by 0.4%. Compared with 45mg/kg nitrobenzene group, vitamin C had no significant change in other indexes except that the SOD in the heart was increased by 11.56%. Conclusion: nitrobenzene has a dose-dependent effect on myocardial mechanical parameters (left ventricular systolic pressure, left ventricular end-diastolic pressure, left ventricular maximal systolic rate and left ventricular maximal diastolic rate), erythrocyte count and SOD,MDA in heart and liver. With the increase of nitrobenzene dose, left ventricular end-diastolic pressure (LVEP), MDA in heart and liver increased gradually, but other indexes decreased gradually. Vitamin C has no obvious antagonism to the damage caused by nitrobenzene.
【学位授予单位】：东北师范大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R96;R363

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