双足鼠脊柱侧凸动物模型的建立及椎体生长板软骨细胞凋亡的表达

发布时间：2018-10-14 18:56

【摘要】：研究背景特发性脊柱侧凸(Idiopathic Scoliosis，以下简称IS)是儿童和青少年最常见的脊椎畸形，其病因仍然不清楚。虽然近年来的研究发现了许多可能的致病因素，如基因遗传因素、生长发育因素、激素的影响、结缔组织病变、肌肉系统的异常、神经系统因素、褪黑素的作用以及血小板的因素等等，但至今国内外对IS的病因认识仍处于朦胧阶段。所以目前还难从病因角度上去预防和治疗。动物模型是病因学研究的重要方法。自从Thillard等报道松果体切除可以在小鸡中诱发出脊柱侧凸的动物模型后，，学者们对松果体以及其分泌的激素一褪黑素在IS中的作用进行了广泛的研究，试图通过褪黑素揭示IS的病因。近年来，Machida等又报道了松果体切除可以在双足鼠中诱发出脊柱侧凸，但此模型侧凸发生机理以及在IS病因研究中的作用还需要进一步研究。另外，IS是一种与生长相关的疾病，只要患者存在生长潜能，侧凸就有进一步加重的危险。而与脊柱生长最直接的相关因素就是椎体的生长板软骨。对于椎体生长板软骨在侧凸发生、发展中作用的研究目前还比较少，而且也面临着在IS病人中取材的困难。本研究试图通过对侧凸动物模型椎体生长板软骨的研究探讨人类IS的病因，并提出IS病因学假说。研究目的验证松果体切除是否能在双足鼠中诱发出脊柱侧凸动物模型并对此模型侧凸发生原因进行探讨。研究该模型椎体生长板软骨细胞的凋亡以及Fas表达在侧凸发生、发展中的作用。
[Abstract]:Background: idiopathic scoliosis (Idiopathic Scoliosis,) is the most common spinal deformity in children and adolescents. Although recent studies have identified a number of possible pathogenic factors, such as genetic factors, growth and development factors, hormone effects, connective tissue lesions, abnormalities of the muscle system, and nervous system factors, The role of melatonin and platelet factors, but the etiology of IS at home and abroad is still in the hazy stage. So at present it is difficult to prevent and treat from the angle of etiology. Animal model is an important method in etiology research. Since Thillard and others reported that pinealectomy can induce scoliosis in chicks, researchers have extensively studied the role of pineal gland and its hormone melatonin in IS. An attempt was made to reveal the etiology of IS by melatonin. In recent years, Machida et al reported that pinealectomy can induce scoliosis in bipedal mice, but the mechanism of scoliosis in this model and its role in the study of IS etiology need further study. In addition, IS is a growth-related disease, as long as the patient has the potential to grow, scoliosis is at risk of further exacerbation. The most direct correlation with the growth of the spine is the growth plate cartilage of the vertebral body. There are few studies on the role of vertebral growth plate cartilage in the development of scoliosis, and it is also difficult to select materials from IS patients. This study attempts to explore the etiology of human IS through the study of vertebrae growth plate cartilage in a contralateral kyphosis animal model, and put forward the IS etiology hypothesis. Objective to investigate whether pinealectomy can induce scoliosis in bipedal mice and to investigate the causes of scoliosis. To study the role of apoptosis and Fas expression in the development of scoliosis.
【学位授予单位】：中国协和医科大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R-332;R682

【相似文献】