HLA-DRB1，DQB1等位基因多态性与长寿的关联研究

发布时间：2018-10-16 14:33

【摘要】： 目的人类白细胞抗原复合体(Human Leukocytes Antigens, HLA)位于人类第6对染色体的短臂上,是一组紧密连锁的基因群,即6P2 .1 .3,全长约3500kb,是人类迄今所知多态性最丰富的基因系统,其信息量约为人类基因组全部信息量的千分之一。传统上,HLA基因分为I类(HLA-A,B, C等),II类(HLA-DR, DQ, DP等)及III类(C2, C4等补体基因)。它们分别编码三类不同的HLA抗原分子,在产物结构、表达方式、组织分布和功能一上各不相同。其中,II类位于D区,为免疫功能相关基因(Immune function related gene, Ir),在免疫应答中起重要的作用。正因为HLA在免疫调节中的重要地位,国外学者对HLA与长寿关系的研究极为重视。已有许多研究表明,长寿与机体的免疫系统功能有直接的联系,提示决定寿限的遗传因素可能存在于控制免疫功能的某些基因上,特别是HLA- II类基因。因此,多数学者都把研究的重点集中于HLA- II类基因,已有较多的长寿与HLA- II类基因关联研究的报道。然而,遗憾的是因人种、民族、地域及研究角度等不同,所得的结论差别很大,甚至得出相反的结果。这正是HLA系统复杂性的表现。国内在这方面的研究与发达国家比较显得滞后得多,目前尚缺乏长寿群体全面、系统的HLA参数。本文通过对大连市内同一地域的长寿人群HLA—DRB1,DQB1等位基因的多态性分布特点研究,旨在探讨长寿人群中HLA-DRB1,DQB1与长寿的关系,为揭开长寿的奥秘提供理论依据。方法应用序列特异的寡核苷酸探针分析法(sequence specific oligonucleotide probes, PCR-SSO)对辽宁省大连市29名90-98岁老人(91±2岁)和随机选取大连地区无偿献血志愿者98名,身体健康,体检标准符合“全国采供血管理规范”。家族无遗传病史,年龄在18-55周岁(38±12)岁,男性49名,女性49名进行HLA-DRB1,DQB1
[Abstract]:Objective the human leukocyte antigen complex (Human Leukocytes Antigens, HLA), located on the short arm of the sixth chromosome, is a closely linked group of genes, I. e., 6P2. 1. 3, with a total length of about 3 500 kb. It is the most polymorphic gene system known to date. The amount of information is about 1/1000 of the total amount of information in the human genome. Traditionally, HLA genes are classified into class I (HLA-A,B, C), II class (HLA-DR, DQ, DP et al.) and III class (C 2, C 4 complement genes). They encode three kinds of different HLA antigenic molecules, which vary in product structure, expression, tissue distribution and function. Among them, II class is located in D region, and (Immune function related gene, Ir), plays an important role in immune response. Because of the important role of HLA in immunomodulation, foreign scholars attach great importance to the relationship between HLA and longevity. Many studies have shown that longevity is directly related to the function of the immune system, suggesting that genetic factors that determine longevity may exist in some genes that control immune function, especially HLA- II genes. Therefore, most scholars have focused on HLA- II genes, and there have been many reports on the association between longevity and HLA- II genes. Unfortunately, however, because of ethnic, regional and research perspective, the conclusions are very different, even the opposite results. This is a manifestation of the complexity of HLA systems. Compared with the developed countries, the domestic research in this field is much lagged behind. At present, there is still a lack of comprehensive and systematic HLA parameters of the longevity population. In this paper, we studied the polymorphism of HLA-DRB1,DQB1 alleles in the longevity population in the same area of Dalian. The aim of this study was to explore the relationship between HLA-DRB1,DQB1 and longevity in the long-lived population, and to provide a theoretical basis for uncovering the mystery of longevity. Methods using sequence specific oligonucleotide probe analysis (sequence specific oligonucleotide probes, PCR-SSO), 29 volunteers aged 90-98 years (91 卤2 years) and 98 volunteers in Dalian, Liaoning province were selected randomly and healthy. The standard of physical examination accords with the National Standard of Blood Collection and supply Management. The family had no history of hereditary disease, aged 18-55 years (38 卤12) years, 49 males and 49 females for HLA-DRB1,DQB1
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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