兔VX2肝癌模型制作的三种方法及其超声评价

发布时间：2018-10-17 17:06

【摘要】：目的:比较三种建立兔VX2肝癌模型方法的效果差异,评价超声在监测兔VX2肝癌中的价值,探讨建立兔VX2肝癌模型的最佳方法。方法:18只新西兰大白兔,随机分为三组:A组超声引导注入肿瘤组织块悬液;B组超声引导经皮穿刺种植肿瘤组织块;C组开腹手术瘤块种植法。分别于接种后第7、14、21和28天采用超声观测三组兔肝脏成瘤情况并记录,处死动物后取病理标本进行对照,比较不同方式肿瘤的生长特点。结果:超声观测下兔VX2肝癌多呈低或等回声结节,边界较清楚,血供较丰富,部分可见声晕。当肿块直径1cm时,超声能准确地反映肿瘤生长情况,与病理检测结果一致。A组多为多结节散在分布,异位种植多;B组肿瘤多生长局限,多突出于肝表面被膜;C组肿瘤呈孤立结节,位于肝实质内。A、B、C三组接种成瘤率分别为:61.1%、44.4%、100%,差异有统计学意义(P0.05)。单发率:11.1%、61.1%、88.9%。异位种植率:55.6%、38.9%、0%。平均存活时间:A组(38.2±4.5)d、B组(37.5±3.6)d、C组(41.2±3.5)d,差异无统计学意义(P0.05)。结论:三种方法均能制成肝癌模型,开腹手术瘤块种植法成功率高、模型性质稳定,是可推荐的建立兔肝癌模型的方法,超声检查是一种监测肝癌模型的有效手段。
[Abstract]:Objective: to compare the effects of three methods for establishing rabbit VX2 liver cancer model, evaluate the value of ultrasound in monitoring rabbit VX2 liver cancer, and explore the best method for establishing rabbit VX2 liver cancer model. Methods: eighteen New Zealand white rabbits were randomly divided into three groups: group A was injected with tumor tissue suspension under ultrasound guidance, group B was guided by percutaneous puncture to implant tumor tissue mass, group C was implanted with tumor mass during open operation. The liver tumorigenesis of the three groups was observed and recorded by ultrasound on the 7th and 28th days after inoculation. The pathological specimens of the rabbits were killed to compare the growth characteristics of the tumors in different ways. Results: the VX2 liver cancer in rabbits showed hypoechoic nodules with clear boundary, abundant blood supply and some acoustical halos. When the tumor diameter was 1cm, ultrasound could accurately reflect the tumor growth, which was consistent with the pathological results. The tumor in group C was solitary nodule located in the liver parenchyma. The rate of inoculation in group C was 61.1% and 44.4%, respectively. The difference was statistically significant (P0.05). Single rate: 11.1% 61.1% 88.9%. Ectopic planting rate: 55.6% and 38.9%. The mean survival time: group A (38.2 卤4.5) days, group B (37.5 卤3.6) days, group C (41.2 卤3.5) days, there was no significant difference (P0.05). Conclusion: all the three methods can be used to make liver cancer model. The success rate of tumor block implantation in open surgery is high and the nature of the model is stable. It is a recommended method to establish rabbit liver cancer model. Ultrasonic examination is an effective method to monitor the liver cancer model.
【作者单位】：哈尔滨医科大学附属肿瘤医院超声科;哈尔滨工业大学计算机科学与技术学院;
【基金】：黑龙江省自然科学基金资助项目(编号:D201170)
【分类号】：R735.7;R445.1;R-332

【参考文献】