同源框基因HOXA11在月经周期人子宫内膜中的表达
发布时间：2018-11-08 15:43
【摘要】： 同源框基因(homeobox gene)是一类胚胎发育的主控基因,这类基因中都存在一个由183个碱基对(bp)组成的、高度保守的同源框(homeobox)序列。HOX基因是同源框基因中的一大类,控制体节的特征,调节中枢神经系统和确定胚胎前后分化关系。在哺乳类HOX基因家族共39个基因,分A、B、C、D四簇位于染色体7,17,12,2的3’端到5’端,分别表达于胚胎的头部到尾部相对应的特定区域。HOX基因表达的蛋白质是一类转录调节因子,都有一个由相应同源框序列编码的约61个氨基酸组成的同源域(homeodomain)结构,这一结构通过与顺式调控元件相结合,实现HOX基因对靶基因的调控。 HOXA11属HOX基因家族,在胚胎尾部体节中表达,主要参与子宫形态的发生和发育。一般而言,在发育期结束后,大部分成年器官不再表达HOX基因,但子宫内膜则是一个例外,因为生育期子宫内膜始终经历着活跃的周期性变化,这种周期性的组织破坏、再构建和重组的过程与胚胎发育过程的调控有某些相似之处,也需要发育调节基因的调控。HOX基因的产物作为转录因子参与调节子宫内膜发育和分化,成为近年来生殖领域的研究热点。 小鼠Hoxa11和人HOXA11分别在小鼠子宫发育,胚泡着床和人的生育期子宫内膜周期性形态发育过程中起着关键作用。Hoxa11基因对小鼠子宫内膜形态发育的调节可直接影响胚泡着床。HOXA11基因在人子宫内膜中也有表达,而且其表达量呈周期性变化,与月经周期中子宫内膜的分化和发育密切相关。HOXA11基因异常表达可能导致子宫不孕、子宫内膜异位症和内膜癌等疾病。目前关于人子宫内膜HOXA11基因的表达,尤其是蛋白质水平的表达规律尚不明了。本课题组曾用原位杂交、RT-PCR等方法对人子宫内膜HOXA11基因的表达进行了一系列研究(国家自然科学基金资助课题39870292、30471814),并首次发现,
[Abstract]:Homobox gene (homeobox gene) is the dominant gene in embryonic development, in which there is a highly conserved homobox (homeobox) sequence composed of 183 base pairs of (bp). HOX gene is a large class of homobox genes. Control the characteristics of somatic ganglia, regulate the central nervous system and determine the relationship between pre-and post-embryonic differentiation. In the mammalian HOX gene family, there are 39 genes, which are located at the 3'to 5'end of chromosome 7171712T2. The proteins expressed in the HOX gene are a class of transcriptional regulatory factors, each having a homologous (homeodomain) structure composed of about 61 amino acids encoded by the corresponding homobox sequence. This structure can regulate the target gene of HOX gene by combining with the cis-regulatory element. HOXA11 belongs to the HOX gene family, expressed in the caudal ganglion of the embryo, mainly involved in the development of uterine morphology. Generally speaking, most adult organs no longer express the HOX gene at the end of the development period, but the endometrium is an exception, because the reproductive endometrium always goes through an active cyclical change, the periodic destruction of the tissue. The process of construction and recombination is similar to the regulation of embryonic development, and the regulation of developmental regulatory gene is also required. The product of HOX gene is involved in the regulation of endometrial development and differentiation as a transcription factor. In recent years, it has become a research hotspot in the field of reproduction. Mouse Hoxa11 and human HOXA11 developed in mouse uterus, The regulation of Hoxa11 gene on the morphological development of mouse endometrium can directly affect the implantation of blastocyst. HOXA11 gene is also expressed in human endometrium. The abnormal expression of HOXA11 gene may lead to infertility, endometriosis and endometrial carcinoma. At present, the expression of human endometrial HOXA11 gene, especially the expression of protein level, is not clear. The expression of HOXA11 gene in human endometrium was studied by in situ hybridization, RT-PCR and other methods (39870292C30471814 funded by the National Natural Science Foundation of China).
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363
本文编号：2318927
[Abstract]:Homobox gene (homeobox gene) is the dominant gene in embryonic development, in which there is a highly conserved homobox (homeobox) sequence composed of 183 base pairs of (bp). HOX gene is a large class of homobox genes. Control the characteristics of somatic ganglia, regulate the central nervous system and determine the relationship between pre-and post-embryonic differentiation. In the mammalian HOX gene family, there are 39 genes, which are located at the 3'to 5'end of chromosome 7171712T2. The proteins expressed in the HOX gene are a class of transcriptional regulatory factors, each having a homologous (homeodomain) structure composed of about 61 amino acids encoded by the corresponding homobox sequence. This structure can regulate the target gene of HOX gene by combining with the cis-regulatory element. HOXA11 belongs to the HOX gene family, expressed in the caudal ganglion of the embryo, mainly involved in the development of uterine morphology. Generally speaking, most adult organs no longer express the HOX gene at the end of the development period, but the endometrium is an exception, because the reproductive endometrium always goes through an active cyclical change, the periodic destruction of the tissue. The process of construction and recombination is similar to the regulation of embryonic development, and the regulation of developmental regulatory gene is also required. The product of HOX gene is involved in the regulation of endometrial development and differentiation as a transcription factor. In recent years, it has become a research hotspot in the field of reproduction. Mouse Hoxa11 and human HOXA11 developed in mouse uterus, The regulation of Hoxa11 gene on the morphological development of mouse endometrium can directly affect the implantation of blastocyst. HOXA11 gene is also expressed in human endometrium. The abnormal expression of HOXA11 gene may lead to infertility, endometriosis and endometrial carcinoma. At present, the expression of human endometrial HOXA11 gene, especially the expression of protein level, is not clear. The expression of HOXA11 gene in human endometrium was studied by in situ hybridization, RT-PCR and other methods (39870292C30471814 funded by the National Natural Science Foundation of China).
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363
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