人肝脏凋亡相关蛋白相互作用蛋白质的规模化发掘

发布时间：2018-11-10 15:37

【摘要】： 凋亡是一个被高度调控的细胞死亡过程,它对保持成熟器官的组织动态平衡具有基础性作用。近年来的大量研究表明,凋亡在大多数细胞中是一个生理上的程序,能被许多外部和内部的信号所触发。许多人类的疾病都直接或者间接的归咎于凋亡的紊乱,凋亡紊乱具体表现为细胞的正常死亡受阻引起的细胞积聚,或者凋亡过度引起的细胞丢失。细胞凋亡的途径主要有两条,一条是通过胞外信号激活细胞内的凋亡酶caspase、一条是通过线粒体释放凋亡酶激活因子激活caspase。这些活化的caspase可将细胞内的重要蛋白降解,引起细胞凋亡。肝脏是人体重要的器官。近年来的研究表明,凋亡在肝脏疾病的发生发展中具有重要的作用。在肝硬变病例的肝细胞、胆管细胞、单核细胞中普遍存在凋亡现象。肝细胞癌形成过程中,不仅癌细胞异常增生,而且突变的细胞凋亡减少,肝癌细胞对凋亡诱导反应明显缺陷。因此凋亡对肝脏正常生理功能的维持,以及肝脏疾病的发生和发展有非常重要的作用。蛋白质-蛋白质相互作用是一切生命活动的基础,揭示相互作用规律是认识生命奥秘的必经之路。酵母双杂交技术是目前应用最成功的大规模研究蛋白质相互作用的技术平台,规模化酵母双杂交技术不是常规实验室酵母双杂交技术在诱饵数量上的简单增加,需要解决大量的技术问题。
[Abstract]:Apoptosis is a highly regulated process of cell death, which plays a fundamental role in maintaining the dynamic balance of mature organs. Recent studies have shown that apoptosis is a physiological process in most cells and can be triggered by many external and internal signals. Many human diseases are directly or indirectly attributed to the disorder of apoptosis, which is characterized by cell accumulation caused by normal cell death or cell loss caused by excessive apoptosis. There are two main pathways of apoptosis, one is to activate the intracellular apoptotic enzyme caspase, through extracellular signal, and the other is to activate caspase. through mitochondrial release of apoptotic enzyme activator. These activated caspase can degrade important proteins in cells and induce apoptosis. The liver is an important organ of the human body. Recent studies have shown that apoptosis plays an important role in the development of liver diseases. Apoptosis is common in hepatocytes, bile duct cells and monocytes in cirrhotic patients. During the formation of hepatocellular carcinoma, not only the abnormal proliferation of cancer cells, but also the apoptosis of mutant cells were decreased, and the apoptosis induced response of hepatoma cells was obviously deficient. Therefore, apoptosis plays an important role in the maintenance of normal physiological function of liver and the occurrence and development of liver diseases. Protein-protein interaction is the basis of all life activities, and revealing the law of interaction is the only way to understand the mystery of life. Yeast two-hybrid technology is the most successful platform for studying protein interaction on a large scale at present. Large-scale yeast two-hybrid technology is not a simple increase in the number of decoys of yeast two-hybrid technology in conventional laboratory. A large number of technical problems need to be solved.
【学位授予单位】：重庆医科大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R341

【共引文献】