小鼠骨髓DC 2-DE技术体系建立及其蛋白质表达分析

发布时间：2018-11-12 09:26

【摘要】： 树突状细胞是机体中功能最强的专职抗原递呈细胞,既能直接激活初始型T淋巴细胞,启动早期特异性免疫应答;又能诱导免疫耐受,具有强大的激活CTL及CD4+ Th细胞的能力,控制着体内免疫反应的过程,在免疫应答中处于中心位置,已成为免疫学及相关学科的研究热点。髓系树突状细胞分化发育的各阶段具有不同的功能特点。未成熟期树突状细胞可由粘附分子选择素介导进入炎症组织,并具有极强的抗原内吞和加工处理能力,但其激活初始T细胞的能力较弱。利用DC来增强机体特异性抗肿瘤免疫能力的研究,已成为肿瘤生物治疗的重要课题。同时,国内外都已将其作为肿瘤疫苗应用于基因治疗,取得了很好的临床效果。如果将抗原引入未成熟树突状细胞,使之发育为成熟细胞,则在临床应用上更为理想。仙台病毒载体是细胞质型RNA载体,对各种动物细胞有较高的感染效率;在理论上不存在改变细胞核内染色体的危险性,与以往的载体相比具有更高的安全性。仙台病毒载体作为基因治疗、基因疫苗、基因(蛋白质)功能分析及蛋白质生产研究的载体,受到许多学者的青睐。本课题对小鼠骨髓来源的未成熟树突状细胞的蛋白质表达进行了分析,通过双向电泳和MALDI-TOF MS分析,运用MS-Fit工具进行鉴定;对仙台病毒载体处理的成熟DC进行比较蛋白质组分析,寻找
[Abstract]:Dendritic cells are the most functional professional antigen presenting cells, which can directly activate the initial T lymphocytes and initiate the early specific immune response. It can induce immune tolerance, have the ability to activate CTL and CD4 Th cells, control the process of immune response in vivo, and play a central role in the immune response. It has become a hot topic in immunology and related disciplines. The differentiation and development of myeloid dendritic cells have different functional characteristics. Immature dendritic cells can be mediated into inflammatory tissue by adhesion molecule selectin, and have strong ability of endocytosis and processing of antigen, but their ability to activate initial T cells is weak. It has become an important subject of tumor biotherapy to use DC to enhance the specific anti-tumor immunity. At the same time, it has been used as tumor vaccine in gene therapy at home and abroad, and achieved good clinical results. If antigen is introduced into immature dendritic cells to develop into mature cells, it is more ideal in clinical application. Sendai virus vector is a cytoplasmic RNA vector with high infection efficiency to various animal cells. In theory, there is no risk of changing the chromosome in the nucleus, which is more safe than the previous vectors. Sendai virus vector has been favored by many scholars as a vector of gene therapy, gene vaccine, gene (protein) function analysis and protein production. The protein expression of immature dendritic cells derived from mouse bone marrow was analyzed in this paper. The expression of proteins was identified by two dimensional electrophoresis and MALDI-TOF MS analysis using MS-Fit tools. Comparative proteome analysis of mature DC treated with Sendai virus vector was carried out to find out
【学位授予单位】：重庆医科大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R392

【共引文献】