细胞凋亡抑制因子survivin在精子发生过程中的作用研究
发布时间：2018-11-13 20:09
【摘要】： 目的:检测细胞凋亡抑制因子survivin mRNA及survivin蛋白在精索静脉曲张大鼠睾丸组织中的表达,初步探讨其在精子发生过程中的意义。 方法:1.利用Saypol法部分结扎大鼠左肾静脉,复制精索静脉曲张大鼠模型,建立生精障碍的动物模型。2.采用逆转录聚合酶链反应(RT-PCR)法及链霉菌抗生物素-过氧化酶连接(SP)免疫组织化学方法检测:(1)精索静脉曲张大鼠睾丸组织中survivin mRNA的表达。(2)精索静脉曲张大鼠睾丸组织中survivin蛋白的表达。 结果:1.精索静脉曲张影响大鼠精子的发生,随着曲张时间的延长,其睾丸损害越重P0.05㖞。2. survivin mRNA及survivin蛋白在正常大鼠睾丸组织中有表达。3. survivin mRNA和survivin蛋白的表达水平与精索静脉曲张的时间有关,曲张的时间越长,survivin在大鼠睾丸组织中的表达越少P0.05㖞。免疫组化显示:survivin蛋白主要表达于次级精母细胞和精子细胞的胞浆,在睾丸间质细胞中有少量表达。4.survivin的表达与睾丸组织的病理分级有关,在减数分裂前出现成熟障碍的睾丸组织中没有表达,而减数分裂后发生成熟障碍的标本的表达量与正常睾丸没有区别。 结论:1.精索静脉曲张是引起精子发生异常的原因之一,与细胞凋亡抑制因子survivin表达密切相关。2. survivin的表达与精子发生早期维持细胞的增殖有关。它可能是精子发生中的一个关键因子。
[Abstract]:Aim: to detect the expression of apoptosis inhibitor survivin mRNA and survivin protein in testis of varicocele rats and to explore their significance in spermatogenesis. Methods: 1. The rat model of varicocele was established by partial ligation of left renal vein by Saypol method. 2. Reverse transcriptase polymerase chain reaction (RT-PCR) and streptomyces biotin-peroxidase (SP) were used to detect the expression of survivin mRNA in testis of varicocele rats. Expression of survivin protein in testis of varicocele rats. The result is 1: 1. Varicocele affects spermatogenesis in rats. With the extension of varicocele, the more serious the testicular damage is? P0.05? 2. Survivin mRNA and survivin protein were expressed in normal rat testis. 3. 3. The expression of survivin mRNA and survivin protein was related to the time of varicocele. The longer the varicocele was, the less survivin was expressed in rat testis. Immunohistochemical staining showed that survivin protein was mainly expressed in the cytoplasm of secondary spermatocytes and spermatocytes, and a little in stromal cells of testis. The expression of 4.survivin was related to the pathological grade of testis. There was no expression in testis with maturation disorder before meiosis, but the expression of maturation disorder after meiosis was not different from that in normal testis. Conclusion: 1. Varicocele is one of the causes of abnormal spermatogenesis, which is closely related to the expression of apoptosis inhibitor survivin. 2. The expression of survivin is related to the proliferation of early spermatogenesis maintenance cells. It may be a key factor in spermatogenesis.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R321.1
本文编号：2330295
[Abstract]:Aim: to detect the expression of apoptosis inhibitor survivin mRNA and survivin protein in testis of varicocele rats and to explore their significance in spermatogenesis. Methods: 1. The rat model of varicocele was established by partial ligation of left renal vein by Saypol method. 2. Reverse transcriptase polymerase chain reaction (RT-PCR) and streptomyces biotin-peroxidase (SP) were used to detect the expression of survivin mRNA in testis of varicocele rats. Expression of survivin protein in testis of varicocele rats. The result is 1: 1. Varicocele affects spermatogenesis in rats. With the extension of varicocele, the more serious the testicular damage is? P0.05? 2. Survivin mRNA and survivin protein were expressed in normal rat testis. 3. 3. The expression of survivin mRNA and survivin protein was related to the time of varicocele. The longer the varicocele was, the less survivin was expressed in rat testis. Immunohistochemical staining showed that survivin protein was mainly expressed in the cytoplasm of secondary spermatocytes and spermatocytes, and a little in stromal cells of testis. The expression of 4.survivin was related to the pathological grade of testis. There was no expression in testis with maturation disorder before meiosis, but the expression of maturation disorder after meiosis was not different from that in normal testis. Conclusion: 1. Varicocele is one of the causes of abnormal spermatogenesis, which is closely related to the expression of apoptosis inhibitor survivin. 2. The expression of survivin is related to the proliferation of early spermatogenesis maintenance cells. It may be a key factor in spermatogenesis.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R321.1
【参考文献】
相关期刊论文 前2条
1 胡传义;;Survivin蛋白在小鼠生精恢复过程中的表达及其与生殖细胞凋亡的关系[J];第四军医大学学报;2006年03期
2 潘连军;高佃军;张鹤鹏;王希友;;精索静脉曲张大鼠生精细胞凋亡与caspase-3蛋白的表达[J];中华男科学杂志;2006年01期
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