大鼠腹膜伤害性刺激c-fos和NOS在中枢神经系统表达的实验研究

发布时间：2018-11-14 07:56

【摘要】：目的:研究大鼠腹膜伤害性刺激c-fos和NOS在中枢神经系统表达。方法:将1%的乙酸注入大鼠腹膜腔造成腹痛膜型,选用健康Wist大鼠56只,雌雄不限随机分为实验组(n=48),对照组(A组n=8),实验组根据实验要求,动物注射乙酸后分别存活30min(n=8)、1h(n=8)、2h(n=8)、4h(n=8)、6h(n=8)和8h(n=8)。依次分为B组、C组、D组、E组、F组、G组。分别取材及切片,用免疫组化ABC法检查中枢内c-fos和NOS阳性神经元的分布。结果:腹膜腔注入乙酸的大鼠,清醒后,表现明显的躁动不安,脊柱向背弓起等。动物被灌注固定后腹腔解剖肉眼可见腹膜充血水肿,大网膜渗出增加,有腹膜炎的病理特征。A组(对照组)FOS少许表达,SPLM统计软件,按α=0.05水准,进行组间t检验,B、C、D、E、F组与A组比较有统计学意义(P0.05),B组与C、D、E组比较有统计学意义(P0.05),D组与E组比较无统计学意义(P0.05),D、E组与G组比较有统计学意义(P0.05),A组与G组比较无统计学意义(P0.05),可推测c-fos阳性神经元呈双侧分布,主要分布于T_6-L_3脊髓后角、延髓孤束核、臂旁核、蓝斑核、中缝背核、下丘脑、室旁核、视上核、杏仁核。NOS表达与对照组相比,向大鼠腹膜腔注射1%的乙酸后主要是1h组、2h组、4h组及6h组的T_6-L_3脊髓后角,延髓孤束核、臂旁核、蓝斑核、中缝背核、下丘脑、室旁核、视上核、杏仁核NOS阳性细胞数均显著增加(P0.01),其与c-fos比较,提前1h到高峰,即大鼠腹膜腔注入乙酸1h NOS表达可达高峰。可知NOS阳性神经元呈双侧分布,也
[Abstract]:Aim: to study the expression of c-fos and NOS in central nervous system (CNS) after noxious peritoneal stimulation in rats. Methods: a total of 1% acetic acid was injected into the peritoneal cavity of rats to induce the type of peritoneal pain. 56 healthy Wist rats were randomly divided into experimental group (n = 48) and control group (n = 8). After acetic acid injection, the animals survived 30min (NN8), 1h (NC8), 2h (NC8), 4h (NC8), 6h (NC8) and 8h (NC8), respectively. B group, C group, D group, E group, F group and G group were divided into two groups. The distribution of c-fos and NOS positive neurons was detected by immunohistochemical ABC method. Results: the rats injected with acetic acid in peritoneal cavity showed obvious restlessness and spinal arch. Peritoneal hyperemia and edema and greater omentum exudation were observed in the naked eye after perfusion and fixation. Group A (control group) showed a little FOS expression, SPLM statistical software, t test between groups according to 伪 = 0. 05 level, B, There was significant difference between), D group and E group (P0.05), D,). There was no significant difference between), D group and E group (P0.05), D,). There was significant difference between group E and group G (P0.05) in), A group and G group (P0.05). It can be inferred that c-fos positive neurons were distributed bilaterally, mainly in the posterior horn of T_6-L_3 spinal cord. The expression of NOS in nucleus solitarius oblongata, parabrachial nucleus, locus coeruleus, dorsal raphe nucleus, hypothalamus, paraventricular nucleus, supraoptic nucleus and amygdala. Compared with the control group, 1% acetic acid was injected into the peritoneal cavity of rats. The number of NOS positive cells in the posterior horn of T_6-L_3 spinal cord, nucleus solitarius oblongata, nucleus parabrachial, nucleus coeruleus, dorsal raphe nucleus, hypothalamus, paraventricular nucleus, supraoptic nucleus and amygdala increased significantly in 4h and 6h groups (P0.01). The expression of NOS reached its peak at 1 h after administration of acetic acid into rat peritoneal cavity. The distribution of NOS positive neurons was bilateral.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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