神经受体在人骨髓间充质干细胞来源的神经细胞上的表达
发布时间：2018-11-18 17:22
【摘要】：目的： 神经细胞移植是中枢神经系统(central nervous system，CNS)退行性疾病、遗传性疾病和中枢神经系统损伤引起的神经系统功能障碍最佳的治疗方案。干细胞作为神经细胞移植的来源，日益受到人们的关注。目前，关于体外诱导骨髓间充质干细胞(bone mesenchymal stem cells，BMSCs)分化为神经细胞的研究报道已有很多，但是，大多数研究是集中于神经细胞标志物，如NSE、NF-M等的检测，神经受体方面的研究鲜有报道。神经受体是神经递质发挥功能所必需的结构，在跨膜信号转导过程中，各种胞膜受体是细胞接受内外环境刺激的门户。不同的化学信号甚至相同的化学信号会因为膜受体的不同而对组织细胞产生截然不同的生理作用。因此，BMSCs诱导分化形成的神经细胞是否表达神经受体对于神经细胞能否发挥正常功能是至关重要的。 多巴胺D2受体(dopamine receptor D2，DRD2)既是突触前受体又是突触后受体，，作为突触前受体可以调节多巴胺的合成和释放，作为突触后受体可以调节DA能神经元的电生理功能。5-羟色胺2(5-hydroxytryptamine 2，5-HT2)受体是突触后受体，能抑制放电活动，主要位于非5-HT能神经元上，是许多抗精神病药物的靶受体。γ-氨基丁酸(gamma-aminobutyric acid，GABA)受体是脑内最普遍的抑制性神经递质受体，其主要的生理作用是调节思维活动，抑制忧虑和焦虑的产生。研究表明DRD2、GABA受体、5-HT受体在阿尔茨海默病(ALzheimer's desease，AD)患者脑内表达下降。 本实验拟利用不同的细胞因子和化学试剂体外诱导人BMSCs向神经细胞分化，然后检测分化形成的神经细胞表达GABA_A受体-α2和α3亚基、5-HT2受体
[Abstract]:Objective: nerve cell transplantation is the best treatment for neurodegenerative diseases, hereditary diseases and neurological dysfunction caused by central nervous system (central nervous system,CNS). Stem cells, as a source of neural cell transplantation, have attracted more and more attention. At present, there have been many reports about inducing the differentiation of bone marrow mesenchymal stem cells (bone mesenchymal stem cells,BMSCs) into nerve cells in vitro. However, most of the studies focus on the detection of neural cell markers, such as NSE,NF-M, etc. Few studies on nerve receptors have been reported. Neuroreceptors are the necessary structures for neurotransmitters to perform their functions. In the transmembrane signal transduction process, various cellular membrane receptors are the gateway for cells to be stimulated by internal and external environment. Different chemical signals and even the same chemical signals have different physiological effects on tissue cells due to different membrane receptors. Therefore, the expression of neuronal receptors in neurons induced by BMSCs is crucial to the normal function of neurons. Dopamine D2 receptor (dopamine receptor D2DRD2 is both a presynaptic receptor and a postsynaptic receptor. As a presynaptic receptor, dopamine synthesis and release can be regulated. As a postsynaptic receptor, it can regulate the electrophysiological function of DA neurons. 5-HT _ 2 receptor is a postsynaptic receptor that inhibits discharge activity and is mainly located in non-5-HT neurons. Gamma-aminobutyric acid (gamma-aminobutyric acid,GABA) receptor is the most common inhibitory neurotransmitter receptor in the brain. Its main physiological function is to regulate the activity of thinking and inhibit the production of anxiety and anxiety. Studies have shown that the expression of DRD2,GABA receptor, 5-HT receptor in the brain of patients with Alzheimer's disease (ALzheimer's desease,AD) decreased. In this study, different cytokines and chemical reagents were used to induce the differentiation of human BMSCs into neural cells in vitro, and then to detect the expression of GABA_A receptor- 伪 2 and 伪 3 subunits and 5-HT2 receptors in the differentiated neurons.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329
本文编号：2340648
[Abstract]:Objective: nerve cell transplantation is the best treatment for neurodegenerative diseases, hereditary diseases and neurological dysfunction caused by central nervous system (central nervous system,CNS). Stem cells, as a source of neural cell transplantation, have attracted more and more attention. At present, there have been many reports about inducing the differentiation of bone marrow mesenchymal stem cells (bone mesenchymal stem cells,BMSCs) into nerve cells in vitro. However, most of the studies focus on the detection of neural cell markers, such as NSE,NF-M, etc. Few studies on nerve receptors have been reported. Neuroreceptors are the necessary structures for neurotransmitters to perform their functions. In the transmembrane signal transduction process, various cellular membrane receptors are the gateway for cells to be stimulated by internal and external environment. Different chemical signals and even the same chemical signals have different physiological effects on tissue cells due to different membrane receptors. Therefore, the expression of neuronal receptors in neurons induced by BMSCs is crucial to the normal function of neurons. Dopamine D2 receptor (dopamine receptor D2DRD2 is both a presynaptic receptor and a postsynaptic receptor. As a presynaptic receptor, dopamine synthesis and release can be regulated. As a postsynaptic receptor, it can regulate the electrophysiological function of DA neurons. 5-HT _ 2 receptor is a postsynaptic receptor that inhibits discharge activity and is mainly located in non-5-HT neurons. Gamma-aminobutyric acid (gamma-aminobutyric acid,GABA) receptor is the most common inhibitory neurotransmitter receptor in the brain. Its main physiological function is to regulate the activity of thinking and inhibit the production of anxiety and anxiety. Studies have shown that the expression of DRD2,GABA receptor, 5-HT receptor in the brain of patients with Alzheimer's disease (ALzheimer's desease,AD) decreased. In this study, different cytokines and chemical reagents were used to induce the differentiation of human BMSCs into neural cells in vitro, and then to detect the expression of GABA_A receptor- 伪 2 and 伪 3 subunits and 5-HT2 receptors in the differentiated neurons.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329
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