大鼠空间辨别性学习记忆时海马结构可塑与受体EphA5的关系

发布时间：2018-11-19 07:20

【摘要】： 目的探讨大鼠获得空间辨别性学习记忆功能时海马结构是否发生形态学可塑性，以及形态可塑性与导向因子EphA5的关系。方法将雄性SD大鼠随机分为模型组、游水组和对照组，模型组又分成7d、14d以及21d三个时间段。模型组用水迷宫训练建立空间辨别性学习记忆动物模型后，用Timm染色方法检测各组以及模型组不同时间海马结构苔藓纤维的出芽。用免疫组织化学方法检测海马结构内受体EphA5阳性细胞的形态与部位以及表达的差异。最后用电镜方法对受体EphA5进行亚细胞定位。实验数据用spsss10.0进行统计学分析。结果 1大鼠经过水迷宫训练的第7d时，其平均潜伏期、运行时间及正确率三项指标都可以达到规定的标准，即平均潜伏期小于5s、运行时间小于15s、正确率大于90％。 2模型组大鼠海马结构内Timm染色显示出芽的神经纤维主要分布在CA3区多形层，并且出芽反应以14d最旺盛，出芽率是88.8％；游水组大鼠的出芽率是22.2％；对照组大鼠均未见苔藓纤维出芽。 3模型组大鼠海马结构CA1区、CA2区、CA3区、CA4区、DG区的EphA5免疫阳性产物的灰度值与游水组、对照组相应亚区相比较低，而免疫阳性细胞计数最多。游水组和对照组对应亚区间的差异没有显著性。在同一组内各亚区间以CA3区、CA4区和DG区内EphA5免疫阳性产物灰度值最低，免疫阳性细胞计数最多。模型组内以14d的灰度值最低，免疫阳性细胞计数最多。 4电镜下受体EphA5的免疫反应阳性产物定位于神经元的胞膜和突触后成份。结论 1．用水迷宫训练大鼠，可以建立空间辨别性学习记忆模型； 2．获得空间辨别性学习记忆功能的大鼠海马结构发生了神经纤维出芽可塑性； 3．受体EphA5免疫反应阳性产物与海马结构神经纤维出芽有关系。
[Abstract]:Objective to investigate whether the hippocampal formation has morphologic plasticity and the relationship between morphological plasticity and EphA5 during the acquisition of spatial discriminant learning and memory function in rats. Methods male SD rats were randomly divided into three groups: model group, swimming group and control group. After the model group was trained with water maze to establish spatial discriminant learning and memory animal model, the sprouting of mossy fibers of hippocampal structure in each group and model group was detected by Timm staining method. The morphology, location and expression of receptor EphA5 positive cells in hippocampal formation were detected by immunohistochemical method. Finally, subcellular localization of receptor EphA5 was performed by electron microscopy. The experimental data were analyzed statistically by spsss10.0. Results 1 at the 7th day of water maze training, the average latency, running time and correct rate of the rats could reach the prescribed standard, that is, the average latency was less than 5 s, and the running time was less than 15 s. The correct rate is more than 90. (2) Timm staining in the hippocampal structure of the model group showed that the budding nerve fibers were mainly distributed in the polymorphic layer of the CA3 region, and the sprout reaction was the most vigorous on the 14th day, and the sprouting rate was 88.8, and the germination rate of the swimming group was 22.2%. No mossy fiber sprouts were found in the control group. 3 the gray values of EphA5 immunoreactive products in the hippocampal CA1, CA2, CA3, CA4 and DG in the model group were lower than those in the swimming group and the control group, but the number of immunopositive cells was the most. There was no significant difference between the swimming group and the control group. In the same group, the gray values of EphA5 immunoreactive products were the lowest in CA3 region, CA4 region and DG region, and the number of immunoreactive cells was the highest. In the model group, the gray value of 14 days was the lowest, and the number of immunoreactive cells was the most. 4 the immunoreactive products of receptor EphA5 were located in the cellular membrane and postsynaptic components of neurons under electron microscope. Conclusion 1. The model of spatial discriminative learning and memory can be established by training rats with water maze. 2. Neural fiber budding plasticity occurred in hippocampal structure of rats with spatial discriminant learning and memory function, 3.The positive product of receptor EphA5 immunoreactive was related to the sprouting of hippocampal nerve fibers.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R322

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