人类精子培养液中能量成分对精子活动指标的影响
发布时间：2018-11-20 15:30
【摘要】： 目的:探讨精子培养液中能量成分的改变对人类精子活力及活率的影响 方法:精液取自自愿供精者,年龄25-40岁,禁欲3-7天,手淫法留取整份精液于无菌杯中,经精液常规分析符合WHO规定的正常标准{2},精浆、血清抗精子抗体均为阴性。以人类输卵管液(Human Tube Fluid,HTF)为基本成分,对HTF配方中的能量成分进行改变作为实验组,以HTF液作为对照组,观察不同配方精子培养液对精子活力(A级精子、B级精子所占精子总数的百分率)、活率(A级精子、B级精子、C级精子所占精子总数的百分率)的影响。不同配方精子培养液分组如下:①实验一:实验组含果糖液(将果糖25mg替代HTF中的葡萄糖)及无糖液(不含糖的HTF)。②实验二:在HTF中加入甘氨酸1.0mM、谷氨酰胺1.0mM、牛磺酸0.1mM。③实验三:在HTF中加入正辛酸0.329mM、正己酸0.38mM取代HTF中的丙酮酸。④实验四:实验组含ADP 2.5mM、含ATP 2.5mM。⑤实验五:实验组含肉毒碱2.5mM及含己酮可可碱2.5mM。每组取10份精液标本,等分为2份或3份,用实验组及对照组培养液进行处理。统计学处理采用SPSS10.0软件包。 结果:①实验一中于培养精子48小时的活力、活率及培养72小时的活力的P值0.05,果糖组优于对照组,对照组优于无糖组。其余时日的动力学指标虽无统计学意义,但果糖组、对照组均优于无糖组。②实验二至实验五中实验组及对照组各项指标P值0.05,差异无统计学意义。 结论:实验表明,在HTF培养液中加入氨基酸、脂肪酸、ADP、ATP、肉毒碱及己酮可可碱均未明显改善精子活力、活率。在实验一中实验组与对照组各项指标无显著差异,但随着培养时间的延长,精子的活力、活率出现差异,果糖组优于对照组,对照组优于无糖组。提示六碳糖是精子的重要供能物质,以果糖代替葡萄糖可显著改善精子的活动能力。
[Abstract]:Objective: to investigate the effect of the changes of energy components in sperm culture medium on the motility and motility of human spermatozoa methods: semen was obtained from volunteers aged 25-40 years, abstinence 3-7 days. The whole semen was collected by masturbation in the aseptic cup. The sperm routine analysis met the normal standard {2} stipulated by WHO. The seminal plasma and serum anti-sperm antibody were all negative. Human oviduct fluid (Human Tube Fluid,HTF) was used as the basic component, the energy composition of HTF formula was changed as the experimental group, and the HTF solution was used as the control group. The sperm motility (A grade spermatozoa) was observed in different formulations of spermatozoa culture medium. The effect of the percentage of B grade spermatozoa on the total number of spermatozoa and the percentage of A grade spermatozoa, B grade sperm and C grade spermatozoa in the total number of spermatozoa. Different formulations of spermatozoa culture fluid were grouped as follows: experiment 1: fructose solution (fructose 25mg was substituted for glucose in HTF) and sugar-free solution (HTF). 2 without sugar); experiment 2: adding glycine 1.0 mm, glutamine 1.0 mm in HTF, glutamine 1.0 mm, Glutamine 1.0 mm. Taurine 0.1mM.3 experiment 3: the addition of n-octanoic acid 0.329mm in HTF and the substitution of pyruvate in HTF by n-caproic acid 0.38mM. 4 experiment 4: the experimental group contains ADP 2.5mM. Experiment 5 with ATP 2.5mM.5: the experimental group contained carnitine 2.5mM and pentoxifylline 2.5 mm. In each group, 10 semen samples were collected and divided into 2 or 3 semen samples, which were treated with the culture medium of the experimental group and the control group. SPSS10.0 software package was used for statistical processing. Results: 1 in experiment 1, the motility and viability of spermatozoa cultured for 48 hours and the activity of 72 hours were 0.05. The fructose group was superior to the control group, and the control group was superior to the sugar-free group. Although there was no statistical significance in the other days, the fructose group and the control group were better than the non-sugar group. 2 from experiment 2 to experiment 5, there was no significant difference between the experimental group and the control group (P 0.05). Conclusion: the addition of amino acids, fatty acids, ADP,ATP, carnitine and pentoxifylline into HTF medium did not significantly improve sperm motility and motility. In experiment 1, there was no significant difference between experimental group and control group, but with the extension of culture time, sperm motility and viability were different. Fructose group was superior to control group, and control group was superior to sugar-free group. It is suggested that hexacarbonose is an important energy supplying substance in spermatozoa. Fructose instead of glucose can significantly improve the motility of spermatozoa.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321-33
本文编号：2345277
[Abstract]:Objective: to investigate the effect of the changes of energy components in sperm culture medium on the motility and motility of human spermatozoa methods: semen was obtained from volunteers aged 25-40 years, abstinence 3-7 days. The whole semen was collected by masturbation in the aseptic cup. The sperm routine analysis met the normal standard {2} stipulated by WHO. The seminal plasma and serum anti-sperm antibody were all negative. Human oviduct fluid (Human Tube Fluid,HTF) was used as the basic component, the energy composition of HTF formula was changed as the experimental group, and the HTF solution was used as the control group. The sperm motility (A grade spermatozoa) was observed in different formulations of spermatozoa culture medium. The effect of the percentage of B grade spermatozoa on the total number of spermatozoa and the percentage of A grade spermatozoa, B grade sperm and C grade spermatozoa in the total number of spermatozoa. Different formulations of spermatozoa culture fluid were grouped as follows: experiment 1: fructose solution (fructose 25mg was substituted for glucose in HTF) and sugar-free solution (HTF). 2 without sugar); experiment 2: adding glycine 1.0 mm, glutamine 1.0 mm in HTF, glutamine 1.0 mm, Glutamine 1.0 mm. Taurine 0.1mM.3 experiment 3: the addition of n-octanoic acid 0.329mm in HTF and the substitution of pyruvate in HTF by n-caproic acid 0.38mM. 4 experiment 4: the experimental group contains ADP 2.5mM. Experiment 5 with ATP 2.5mM.5: the experimental group contained carnitine 2.5mM and pentoxifylline 2.5 mm. In each group, 10 semen samples were collected and divided into 2 or 3 semen samples, which were treated with the culture medium of the experimental group and the control group. SPSS10.0 software package was used for statistical processing. Results: 1 in experiment 1, the motility and viability of spermatozoa cultured for 48 hours and the activity of 72 hours were 0.05. The fructose group was superior to the control group, and the control group was superior to the sugar-free group. Although there was no statistical significance in the other days, the fructose group and the control group were better than the non-sugar group. 2 from experiment 2 to experiment 5, there was no significant difference between the experimental group and the control group (P 0.05). Conclusion: the addition of amino acids, fatty acids, ADP,ATP, carnitine and pentoxifylline into HTF medium did not significantly improve sperm motility and motility. In experiment 1, there was no significant difference between experimental group and control group, but with the extension of culture time, sperm motility and viability were different. Fructose group was superior to control group, and control group was superior to sugar-free group. It is suggested that hexacarbonose is an important energy supplying substance in spermatozoa. Fructose instead of glucose can significantly improve the motility of spermatozoa.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321-33
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