MicroRNA-150通过降低CD122的表达调控NK及NKT细胞的分化

发布时间：2018-11-21 10:33

【摘要】：目的:探讨microRNA-150缺失对调节性T细胞(Treg)、γδT细胞、NK及NKT细胞产生、发育和自稳的影响。方法:采用microRNA-150基因敲除小鼠;Real-time PCR检测microRNA-150的表达;流式细胞术检测小鼠胸腺和脾脏Treg、γδT细胞、NK及NKT细胞数量变化;采用Annexin V染色法检测细胞凋亡;5-溴脱氧尿嘧啶核苷(Brdu)掺入法检测细胞增殖。结果:microRNA-150缺失对小鼠Treg和γδT细胞的产生和发育不发挥明显作用,但导致NK和胸腺NKT细胞数量显著降低,而且,microRNA-150缺失导致小鼠NK及NKT细胞中CD122的表达显著降低,细胞发育受阻,凋亡增加,同时NK细胞的增殖能力也显著降低。结论:microRNA-150调控小鼠NK和NKT细胞的发育和自稳,CD122可能在以上调控过程中发挥重要作用。
[Abstract]:Aim: to investigate the effects of microRNA-150 deletion on the production, development and autostability of (Treg), 纬未 T cells, NK and NKT cells in regulatory T cells. Methods: microRNA-150 knockout mice, microRNA-150 expression were detected by Real-time PCR, Treg, 纬未 T cells, NK and NKT cells in thymus and spleen were detected by flow cytometry, apoptosis was detected by Annexin V staining. Cell proliferation was detected by 5-bromodeoxyuridine (Brdu) incorporation. Results: microRNA-150 deletion did not play a significant role in the production and development of Treg and 纬未 T cells, but resulted in a significant decrease in the number of NK and thymus NKT cells, and a significant decrease in the expression of CD122 in NK and NKT cells. Cell development was blocked, apoptosis was increased, and proliferation of NK cells was significantly decreased. Conclusion: microRNA-150 regulates the development and homeostasis of NK and NKT cells in mice, and CD122 may play an important role in the regulation.
【作者单位】：唐山市工人医院;河北联合大学;
【基金】：国家自然科学基金面上项目(No.81373111) 河北省自然科学基金面上项目(No.H2013209019) 唐山市科技局指令性项目(No.13130290z)资助
【分类号】：R363

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