河北卢龙县猪与人G3型A组轮状病毒进化关系研究

发布时间：2018-11-22 09:40

【摘要】：对存于中国疾病控制预防控制中心病毒病预防控制所病毒性腹泻室的一例卢龙G3型仔猪GARV LLZ212的主要基因片段VP7、VP4、VP6、VP2和NSP2-5进行扩增,确定其基因型,并探讨其与当地人G3型GARV的进化关系。采用逆转录-聚合酶链式反应(Reverse transcription-polymerase chain reaction,RT-PCR),对LLZ212标本及6例当地儿童G3型GARV阳性标本的VP7、VP4、VP6、VP2和NSP2-5进行扩增,并进行序列测定。应用A组轮状病毒自动分型工具(RotaC v2.0)对各个片段进行分型。应用DNAStar和MEGA5.0软件包与GenBank上的参考株进行同源性和系统进化分析。卢龙猪LLZ212基因型为G3-P[8]-I5-C1-N1-T1-E1-H1,6株人GARV基因型均为G3-P[8]-I1-C1-N1-T1-E1-H1。LLZ212基因片段VP7、VP4、NSP4和NSP5分别与人GARV E885、CMH014/07、Wa和RMC321株同源性最高,且进化树在同一亚枝内;LLZ212基因片段VP6、NSP2和NSP3均与猪GARV PRG921株同源性最高,基因片段VP2与猪GARV OSU株同源性最高,且进化树在同一亚枝内。确定一株罕见G3-P[8]-I5-C1-N1-T1-E1-H1型猪GARV,可能为人-猪重配株,LLZ212基因片段VP7、VP4、NSP4和NSP5与人GARV同源性最高,LLZ212基因片段VP6、VP2、NSP2和NSP3猪GARV同源性最高。
[Abstract]:The main gene fragments VP7,VP4,VP6,VP2 and NSP2-5 of GARV LLZ212 of Lulong G3 piglets were amplified and their genotypes were determined by amplification of VP7,VP4,VP6,VP2 and NSP2-5 in the virus diarrhea laboratory of the Central Center for Disease Control and Prevention of China. The evolutionary relationship between G3 GARV and local people was also discussed. Reverse transcription-polymerase chain reaction (Reverse transcription-polymerase chain reaction,RT-PCR) was used to amplify VP7,VP4,VP6,VP2 and NSP2-5 from LLZ212 samples and G3 GARV positive samples from 6 local children. Group A rotavirus automatic typing tool (RotaC v2.0) was used to type each fragment. The homology and phylogenetic analysis of reference strains on GenBank were carried out by using DNAStar and MEGA5.0 software packages. The LLZ212 genotypes of Lulong pigs were G3-P [8] -I5-C1-N1-T1-E1-H1G, all of which were G3-P [8]-I1-C1-N1-T1-E1-H1.LLZ212 gene fragment VP7,VP4,NSP4 and NSP5, respectively. Wa and RMC321 have the highest homology, and the evolutionary tree is in the same sub-branch. The VP6,NSP2 and NSP3 fragments of LLZ212 gene were most homologous to GARV PRG921 strain, and VP2 gene fragment was the most homologous to GARV OSU strain, and the phylogenetic tree was in the same subbranch. It was confirmed that a rare G3-P [8]-I5-C1-N1-T1-E1-H1 porcine GARV, might be a human-to-pig rematch strain. The LLZ212 gene fragments VP7,VP4,NSP4 and NSP5 share the highest homology with human GARV, and LLZ212 gene fragment VP6,VP2,. NSP2 and NSP3 porcine GARV have the highest homology.
【作者单位】：中国疾病控制预防控制中心病毒病预防控制所病毒性腹泻室;兰州大学第一临床医学院儿科;宁夏医科大学检验学院;
【分类号】：R373

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