镉对雄性大鼠脊髓下腰段运动神经元的影响

发布时间：2018-11-24 12:56

【摘要】：目的研究镉对成年雄性大鼠脊髓下腰段SNB和DLN运动神经元超微结构和雄激素受体(AR)表达的影响。方法成年雄性大鼠腹腔内反复小剂量注射氯化镉(0.5mg/kg体重和1.0mg/kg体重交替注射)及丙酸睾酮(TP)500μg皮下注射后,采血测定血清睾酮含量,取脊髓L5-6节段进行SNB和DLN运动神经元AR免疫组化及光密度(OD)分析,透射电镜观察SNB和DLN运动神经元超微结构,采用原子吸收石墨炉法测定脊髓镉含量。结果染镉后脊髓内镉含量升高,血清睾酮水平在染镉30d时明显下降。染镉7d、10d、15d,神经元核AR免疫染色平均OD值降低,TP皮下注射2h,AR平均OD值无明显升高;染镉30d,AR平均OD值进一步降低,TP皮下注射2h,AR平均OD值增加,但是只达到单纯染镉7d、10d、15d时水平。染镉后,神经元超微结构无明显改变。结论镉可以蓄积在成年大鼠脊髓内,导致SNB和DLN运动神经元功能损伤,可能与镉所致性功能障碍有关。
[Abstract]:Objective to study the effects of cadmium on the ultrastructure of SNB and DLN motor neurons and the expression of androgen receptor (AR) in the lower lumbar spinal cord of adult male rats. Methods male adult rats were injected with cadmium chloride (0.5mg/kg body weight and 1.0mg/kg body weight alternately) and testosterone propionate (TP) 500 渭 g) subcutaneously injected intraperitoneally. Serum testosterone levels were measured. The spinal cord L5-6 segments were taken for AR immunohistochemistry and optical density (OD) analysis of SNB and DLN motor neurons. The ultrastructure of SNB and DLN motor neurons was observed by transmission electron microscope. The content of CD in spinal cord was measured by atomic absorption graphite furnace method. Results the content of CD in spinal cord increased after cadmium exposure, and the serum testosterone level decreased significantly after 30 days of cadmium exposure. The mean OD value of AR immunostaining in neurons decreased after 7 days and 10 days and 15 days after exposure to cadmium, but the average OD value of AR was not significantly increased at 2 hours after subcutaneous injection of TP. After 30 days of cadmium exposure, the average OD value of AR decreased further, and the average OD value of TP increased at 2 h after subcutaneous injection, but only reached the level of 10 days or 15 days after exposure to cadmium alone for 7 days or 10 days. The ultrastructure of neurons did not change after cadmium exposure. Conclusion cadmium accumulates in the spinal cord of adult rats and results in functional damage of SNB and DLN motor neurons, which may be related to the sexual dysfunction caused by cadmium.
【作者单位】：重庆医科大学电子显微镜室重庆医科大学电子显微镜室
【分类号】：R114

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