大肠杆菌多重耐药负性调节基因及膜孔蛋白的研究
发布时间：2018-11-25 14:03
【摘要】： 为了研究大肠杆菌多重耐药操纵子抑制基因MarR及大肠杆菌外输泵抑制基因AcrR突变,和膜孔蛋白缺失与大肠杆菌多重耐药的关系。本实验对标准菌株、临床分离的敏感菌株及进行人工诱导的耐药菌株和临床分离的耐药菌株,从基因水平和蛋白水平进行了研究,通过对MarR和AcrR基因的克隆测序和膜孔蛋白的提取,来分析大肠杆菌多重耐性与MarR和AcrR突变及膜孔蛋白缺失的关系。结果表明,耐药株与敏感株的MarR和AcrR均有不同程度的突变,MarR基因突变对细菌多重耐药的影响较小,AcrR基因突变可能更大程度的导致细菌对多重抗生素耐药的产生。只有一株耐药菌发现缺失膜孔蛋白OmpF,推测膜孔蛋白对大肠杆菌多重耐药的影响较小。
[Abstract]:In order to study the relationship between multidrug resistance of Escherichia coli (E. coli) multidrug resistance gene (MarR), Escherichia coli pump suppressor gene (AcrR) mutation, membrane pore protein deletion and multidrug resistance of Escherichia coli. In this study, the standard strain, the sensitive strain isolated in clinic, the drug-resistant strain induced by artificial and the drug-resistant strain isolated in clinic were studied from the gene level and protein level. Through cloning and sequencing of MarR and AcrR genes and extraction of membrane porin, the relationship between multiple tolerance of Escherichia coli and MarR and AcrR mutation and membrane pore protein deletion was analyzed. The results showed that the MarR and AcrR of resistant and sensitive strains were mutated to different degrees. The mutation of MarR gene had little effect on the multidrug resistance of bacteria. The mutation of AcrR gene might lead to the production of multidrug resistance of bacteria to multiple antibiotics to a greater extent. Only one strain of drug-resistant bacteria found that the absence of membrane pore protein (OmpF,) had little effect on the multidrug resistance of Escherichia coli.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：S852.61;R378
本文编号：2356372
[Abstract]:In order to study the relationship between multidrug resistance of Escherichia coli (E. coli) multidrug resistance gene (MarR), Escherichia coli pump suppressor gene (AcrR) mutation, membrane pore protein deletion and multidrug resistance of Escherichia coli. In this study, the standard strain, the sensitive strain isolated in clinic, the drug-resistant strain induced by artificial and the drug-resistant strain isolated in clinic were studied from the gene level and protein level. Through cloning and sequencing of MarR and AcrR genes and extraction of membrane porin, the relationship between multiple tolerance of Escherichia coli and MarR and AcrR mutation and membrane pore protein deletion was analyzed. The results showed that the MarR and AcrR of resistant and sensitive strains were mutated to different degrees. The mutation of MarR gene had little effect on the multidrug resistance of bacteria. The mutation of AcrR gene might lead to the production of multidrug resistance of bacteria to multiple antibiotics to a greater extent. Only one strain of drug-resistant bacteria found that the absence of membrane pore protein (OmpF,) had little effect on the multidrug resistance of Escherichia coli.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：S852.61;R378
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