脐血间充质干细胞抑制外周血淋巴细胞增殖的作用

发布时间：2018-12-11 12:29

【摘要】：目的研究脐血间充质干细胞(UCBMSC)对成人外周血淋巴细胞增殖的影响。方法分离、扩增UCBMSC并鉴定其表面标志及向成骨细胞及脂肪细胞的分化能力。UCBMSC与淋巴细胞直接共培养并用植物血凝素(PHA)刺激,或在TranswellTM非接触体系中,用PHA刺激共培养。两个培养体系均分为阴性对照组(淋巴细胞单独培养组)、阳性对照组(淋巴细胞加PHA刺激组)、实验组(淋巴细胞加PHA和UCBMSC组)。四氮唑衍生物MTS还原法测定细胞增殖并计算抑制率。结果在共培养与TranswellTM非接触体系中不同数量UCBMSC对淋巴细胞增殖均具有抑制作用,且UCBMSC浓度越高,抑制作用越强(P0.05),具有剂量依赖性。但两体系中UCBMSC与淋巴细胞比值相同时,共培养体系抑制作用较非接触体系强,且差异具有统计学意义(P0.05)。结论 UCBMSC能抑制淋巴细胞增殖,且共培养体系抑制作用较非接触体系强。
[Abstract]:Objective to study the effect of umbilical cord blood mesenchymal stem cell (UCBMSC) on the proliferation of adult peripheral blood lymphocytes. Methods UCBMSC was isolated, amplified, and its surface markers and differentiation ability into osteoblasts and adipocytes were identified. UCBMSC was co-cultured with lymphocytes and stimulated with phytohemagglutinin (PHA) or co-cultured with PHA in TranswellTM non-contact system. The two culture systems were divided into negative control group (lymphocyte culture group), positive control group (lymphocyte plus PHA stimulation group) and experimental group (lymphocyte plus PHA and UCBMSC group). The cell proliferation was measured by MTS reduction method and the inhibitory rate was calculated. Results different amounts of UCBMSC could inhibit the proliferation of lymphocytes in non-contact system of co-culture and TranswellTM, and the higher the concentration of UCBMSC was, the stronger the inhibition was (P0.05) in a dose-dependent manner. But when the ratio of UCBMSC and lymphocyte in the two systems was the same, the inhibition effect of co-culture system was stronger than that of non-contact system, and the difference was statistically significant (P0.05). Conclusion UCBMSC can inhibit lymphocyte proliferation, and the co-culture system has stronger inhibitory effect than non-contact system.
【作者单位】：宁波大学医学院附属宁波市第一医院;
【基金】：宁波市社会发展科研项目(2010C50019)
【分类号】：R329

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