声诱发短潜伏期负电位（ASNR）的豚鼠模型
发布时间：2018-12-12 06:43
【摘要】： 目的:ASNR为强声刺激出现的前庭源性电位,重度感音性聋及球囊功能正常耳为其出现的必备条件。本研究通过验证豚鼠ASNR与VEMP出现的一致性来建立ASNR豚鼠模型,即建立重度感音性聋且球囊功能正常的豚鼠,用短声刺激诱发ASNR。 方法:将27只健康豚鼠随机分为两组,正常对照组16只(32耳)、给药致聋组11只(22耳)。致聋组经给药(400mg/kg Ka皮下注射,一小时后,40mg/kg Ea颈静脉注射)致聋后至少恢复一周。对所有动物进行听觉前庭功能检查包括(ABe测试、VEMP测试及冰水试验)。致聋组豚鼠根据ASNR的出现分为两组(ASNR组、非ASNR组)。 结果:11只豚鼠(22耳)中8耳出现ASNR,出现率为36.4%,其阈值范围为120-130dB SPL(平均124.4±4.96dB SPL),潜伏期(1.75ms-2.60ms),平均为2.15±0.27ms,阈值平均潜伏期2.34±0.18ms。ASNR组8耳皆出现VEMP,VEMP阈值及正负峰潜伏期与正常组比较均无统计学差异,未出现VEMP的10耳,皆无ASNR,非ASNR组中有四耳出现VEMP,其阈值及正负峰潜伏期与正常组比较亦无统计学差异。致聋组VEMP、ASNR的引出与冰水诱发正常眼震均无相关关系。 结论:实验应用前庭球囊测试指标(VEMP)验证了ASNR的出现与其具有一致性,从而证明了ASNR与VEMP同起源于球囊,而与冰水试验结果无关。本实验在重度感音性聋且球囊功能正常的豚鼠身上诱发出ASNR,通过耳毒药物法首次成功建立ASNR动物模型。
[Abstract]:Objective: ASNR is the vestibular potential of strong sound stimulation, and severe sensorineural hearing loss and normal balloon function are the necessary conditions. The aim of this study was to establish a guinea pig model of ASNR by verifying the consistency between ASNR and VEMP, that is, to establish a guinea pig with severe sensorineural hearing loss and normal balloon function. ASNR. was induced by short sound stimulation. Methods: 27 healthy guinea pigs were randomly divided into two groups: the control group (n = 16) (n = 16) and the drug induced deafness group (n = 11) (n = 22). The deafness group recovered at least one week after administration of 400mg/kg Ka (subcutaneous injection, 1 hour later, 40mg/kg Ea via jugular vein). Auditory vestibular tests were performed on all animals (ABe test, VEMP test and ice water test). According to the appearance of ASNR, guinea pigs in deafness group were divided into two groups (ASNR group, non-ASNR group). Results: in 11 guinea pigs (22 ears), the occurrence rate of ASNR, in 8 ears was 36.4, and the threshold value was 120-130dB SPL (mean 124.4 卤4.96dB SPL), latency (1.75ms-2.60ms), average 2.15 卤0.27 Ms). There was no significant difference in VEMP,VEMP threshold and positive and negative peak latency in 8 ears of the group with 2. 34 卤0.18ms.ASNR, 10 ears without VEMP, and 4 ears with no ASNR, in non ASNR group. There was no significant difference in the threshold and latency of positive and negative peaks between the two groups. There was no correlation between VEMP,ASNR elicitation and normal nystagmus induced by ice water in deafness group. Conclusion: the vestibular balloon test index (VEMP) was used to verify the consistency between the appearance of ASNR and ASNR, which proved that both ASNR and VEMP originated from the balloon, but had nothing to do with the results of ice water test. In this study, ASNR, was successfully established in guinea pigs with severe sensorineural deafness and normal balloon function by ototoxic method.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R764;R-332
本文编号：2374115
[Abstract]:Objective: ASNR is the vestibular potential of strong sound stimulation, and severe sensorineural hearing loss and normal balloon function are the necessary conditions. The aim of this study was to establish a guinea pig model of ASNR by verifying the consistency between ASNR and VEMP, that is, to establish a guinea pig with severe sensorineural hearing loss and normal balloon function. ASNR. was induced by short sound stimulation. Methods: 27 healthy guinea pigs were randomly divided into two groups: the control group (n = 16) (n = 16) and the drug induced deafness group (n = 11) (n = 22). The deafness group recovered at least one week after administration of 400mg/kg Ka (subcutaneous injection, 1 hour later, 40mg/kg Ea via jugular vein). Auditory vestibular tests were performed on all animals (ABe test, VEMP test and ice water test). According to the appearance of ASNR, guinea pigs in deafness group were divided into two groups (ASNR group, non-ASNR group). Results: in 11 guinea pigs (22 ears), the occurrence rate of ASNR, in 8 ears was 36.4, and the threshold value was 120-130dB SPL (mean 124.4 卤4.96dB SPL), latency (1.75ms-2.60ms), average 2.15 卤0.27 Ms). There was no significant difference in VEMP,VEMP threshold and positive and negative peak latency in 8 ears of the group with 2. 34 卤0.18ms.ASNR, 10 ears without VEMP, and 4 ears with no ASNR, in non ASNR group. There was no significant difference in the threshold and latency of positive and negative peaks between the two groups. There was no correlation between VEMP,ASNR elicitation and normal nystagmus induced by ice water in deafness group. Conclusion: the vestibular balloon test index (VEMP) was used to verify the consistency between the appearance of ASNR and ASNR, which proved that both ASNR and VEMP originated from the balloon, but had nothing to do with the results of ice water test. In this study, ASNR, was successfully established in guinea pigs with severe sensorineural deafness and normal balloon function by ototoxic method.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R764;R-332
【引证文献】
相关硕士学位论文 前1条
1 李君梅;电针耳穴对豚鼠老年性聋耳蜗核、下丘和听皮层中SOD表达的影响[D];泸州医学院;2011年
，本文编号：2374115
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