登革Ⅱ型病毒E蛋白基因原核及真核表达质粒的构建
发布时间：2018-12-12 13:02
【摘要】：研究的背景及目的： 登革病毒(Dengue virus，DV)是黄病毒科的单股正链RNA病毒，有4种血清型，均可以引起登革热(Classical dengue fever，DF)和登革出血热／登革休克综合征(Dengue hemorrhagic fever／Dengue shock syndrome，DHF／DSS)，广泛流行于热带和亚热带地区。每年DF病例超过1亿，DHF病例约50万。WHO已将DHF／DSS、肝炎、疟疾、结核列为全球流行最严重的传染病。但DV的发病机理不明，临床治疗也主要是支持疗法和对症疗法对症为主。近年来，在世界范围内和我国南方以及东南亚，DHF／DSS的发病率有明显增加的趋势，可见登革病毒的感染已是严重的公共卫生问题。 关于DHF／DSS发病机制的看法，主要有三种：①抗体依赖增强(Antibody-dependent enhancement，ADE)理论：认为DV特异性IgG所引起的免疫增强效应导致出现DHF／DSS的病理变化；②病毒因素致病理论：认为病毒毒力或复制能力等因素是导致严重疾病的原因；③免疫病理反应理论：认为交叉反应性T细胞介导的细胞免疫损伤是DHF／DSS的主要病理机制。尽管传统的减毒活疫苗已进行多年的临床试验，但是到目前为止，仍没有安全有效的登革疫苗用于临床。因此，登革新型疫苗的研究极为迫切和重要。 DEN基因组为单股正链RNA，约11kb，从5’到3’端排列着3个结构基因(C、prM和E)和7个非结构基因(NS1、NS2A、NS2B、NS3、NS4A、NS4B和NS5)，分别编码相应的蛋白。结构基因(占病基因组20％以上)影响着病毒的组装，进入宿主细胞和免疫反应。E蛋白是DV病毒体上包膜糖蛋白与最大的结构蛋白，由495个氨基酸组成，
[Abstract]:Background and objective: dengue virus (Dengue virus,DV) is a single-stranded positive strand RNA virus of the family Flavoviridae. There are four serotypes, all of which can cause dengue (Classical dengue fever,. DF) and dengue hemorrhagic fever / dengue shock syndrome (Dengue hemorrhagic fever/Dengue shock syndrome,DHF/DSS), widely prevalent in tropical and subtropical regions. There are more than 100 million DF cases and about 500000 DHF cases each year. WHO has listed DHF/DSS, hepatitis, malaria and tuberculosis as the most serious infectious diseases in the world. However, the pathogenesis of DV is not clear, and the clinical treatment is mainly supportive therapy and symptomatic therapy. In recent years, the incidence of DHF/DSS has increased significantly in the world and in the south of China and Southeast Asia. It can be seen that dengue virus infection is a serious public health problem. There are three main views on the pathogenesis of DHF/DSS: (1) the theory of antibody dependent enhancement (Antibody-dependent enhancement,ADE): it is believed that the immunological enhancement induced by DV specific IgG leads to the pathological changes of DHF/DSS; (2) the virulence or replication ability of virus was considered as the cause of severe disease, and the immunopathologic reaction theory: it was considered that the main pathological mechanism of DHF/DSS was the cellular immune damage mediated by cross-reactive T cells. Although traditional live attenuated vaccines have been tested for many years, there is no safe and effective dengue vaccine for clinical use. Therefore, the research of innovative vaccine is extremely urgent and important. The DEN genome is a single-stranded positive strand RNA, of about 11 kb. From 5'to 3 'end, there are three structural genes (CCM and E) and seven non-structural genes (NS1,NS2A,NS2B,NS3,NS4A,NS4B and NS5), which encode the corresponding proteins respectively. Structural genes (accounting for more than 20% of the diseased genome) affect the assembly of the virus, entering host cells and immune responses. E protein is the largest structural protein and envelope glycoprotein on the DV virus, which consists of 495 amino acids.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373
本文编号：2374606
[Abstract]:Background and objective: dengue virus (Dengue virus,DV) is a single-stranded positive strand RNA virus of the family Flavoviridae. There are four serotypes, all of which can cause dengue (Classical dengue fever,. DF) and dengue hemorrhagic fever / dengue shock syndrome (Dengue hemorrhagic fever/Dengue shock syndrome,DHF/DSS), widely prevalent in tropical and subtropical regions. There are more than 100 million DF cases and about 500000 DHF cases each year. WHO has listed DHF/DSS, hepatitis, malaria and tuberculosis as the most serious infectious diseases in the world. However, the pathogenesis of DV is not clear, and the clinical treatment is mainly supportive therapy and symptomatic therapy. In recent years, the incidence of DHF/DSS has increased significantly in the world and in the south of China and Southeast Asia. It can be seen that dengue virus infection is a serious public health problem. There are three main views on the pathogenesis of DHF/DSS: (1) the theory of antibody dependent enhancement (Antibody-dependent enhancement,ADE): it is believed that the immunological enhancement induced by DV specific IgG leads to the pathological changes of DHF/DSS; (2) the virulence or replication ability of virus was considered as the cause of severe disease, and the immunopathologic reaction theory: it was considered that the main pathological mechanism of DHF/DSS was the cellular immune damage mediated by cross-reactive T cells. Although traditional live attenuated vaccines have been tested for many years, there is no safe and effective dengue vaccine for clinical use. Therefore, the research of innovative vaccine is extremely urgent and important. The DEN genome is a single-stranded positive strand RNA, of about 11 kb. From 5'to 3 'end, there are three structural genes (CCM and E) and seven non-structural genes (NS1,NS2A,NS2B,NS3,NS4A,NS4B and NS5), which encode the corresponding proteins respectively. Structural genes (accounting for more than 20% of the diseased genome) affect the assembly of the virus, entering host cells and immune responses. E protein is the largest structural protein and envelope glycoprotein on the DV virus, which consists of 495 amino acids.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373
【引证文献】
相关硕士学位论文 前1条
1 刘新鑫;APMV-1 F基因表达及复合间接ELISA方法的建立与应用[D];吉林大学;2008年
，本文编号：2374606
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