季节性流感疫苗免疫血清与H7N9禽流感病毒交叉反应

发布时间：2018-12-12 20:06

【摘要】：目的分析接种季节性流感疫苗人群是否具有对新发H7N9流感病毒具有交叉保护,并反向检测了雪貂产生H7N9抗血清与H1/H3/H5不同亚型流感病毒的免疫反应。方法利用血凝抑制试验和微量中和实验检测疫苗免疫后人群转阳血清对A/Anhui/1(H7N9)病毒中和作用及雪貂H7N9抗血清与A/California/07/2009(H1N1)、A/PR/8/34(H1N1)、A/Brisbane/59/2007(H1N1)、A/Brisbane/10/2007(H3N2)、A/Shenzhen/406H/2006(H5N1)和A/Vietnam/1203/2004(H5N1)等不同亚型病毒的中和作用。结果季节性流感疫苗受众者转阳血清与H7N9病毒反应HI及中和抗体反应均为阴性,雪貂H7N9抗血清与H1/H3/H5不同亚型流感病毒反应HI及中和抗体反应均为阴性。结论季节性流感疫苗接种人群不具有对新发H7N9流感病毒交叉保护,并且反向验证了H7N9病毒抗血清与H1/H3/H5亚型流感病毒亦无交叉免疫反应。
[Abstract]:Objective to analyze the cross-protection of seasonal influenza vaccine against new H7N9 influenza virus, and to detect the immune response of ferret to H7N9 antiserum and H1/H3/H5 subtype influenza virus. Methods Hemagglutination inhibition test and micro neutralization test were used to detect the neutralization of A/Anhui/1 (H7N9) virus and ferret H7N9 antiserum with A/California/07/2009 (H1N1), A/PR/8/34 (H1N1). Neutralization of A/Brisbane/59/2007 (H1N1), A/Brisbane/10/2007 (H3N2), A/Shenzhen/406H/2006 (H5N1) and A/Vietnam/1203/2004 (H5N1) subtypes. Results the negative reaction of HI and neutralizing antibody against H7N9 virus was found in seasonal influenza vaccine recipients, and the reaction of HI and neutralizing antibody in ferret H7N9 antiserum with different subtypes of H1/H3/H5 was negative. Conclusion seasonal influenza vaccination population does not have cross-protection against new H7N9 influenza virus, and there is no cross immune reaction between H7N9 antiserum and H1/H3/H5 subtype influenza virus.
【作者单位】：中国医学科学院北京协和医学院医学实验动物研究所卫生部人类疾病比较医学重点实验室国家中医药管理局人类疾病动物模型三级实验室;
【基金】：十二五科技重大专项(2012ZX10004501-004-003,2012ZX10004501-004-004,2012ZX10004301-8) 国家自然科学基金(31370203) 科技部H7N9禽流感应急防控专项(KJYJ-2013-01-04)
【分类号】：R392.1

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