融合表达ESAT6-CFP10结核病疫苗的构建及其免疫学特性研究
发布时间：2018-12-13 04:05
【摘要】：结核病(Tuberculosis,TB)是由结核分枝杆菌(Mycobacterium tuberculosis,MTB)所致以呼吸系统感染为主的慢性传染病,据WHO估计,目前全世界约有1/3人口感染MTB,每年有1000万新发患者和300万患者死亡。贫穷、人口增加、流动人口增多、耐药MTB增多及艾滋病流行等使TB问题雪上加霜。我国是全球22个TB高负担国家之一,现有MTB感染人数已达4亿,传染性TB患者达到200万。卡介苗(BCG)虽被广泛用于预防TB,但其效果仍不够满意。研究认为BCG可预防并减轻儿童的严重TB,但对成人TB的预防作用从0到80%不等。导致此现象的原因在于BCG菌株的变异使保护性抗原丢失、人群间遗传或营养的差异及环境等因素的影响。鉴于BCG自身的不足,以及当前TB流行的严重性,研究新的疫苗用于TB的预防已成为当前国内外研究热点。 MTB早期培养滤液蛋白(CFP)中的ESAT6和CFP10是重要的保护性抗原。本研究先后构建了融合表达ESAT6和CFP10蛋白的亚单位疫苗、基因疫苗以及重组耻垢分枝杆菌(mycobacterium smegmatis,M.S)疫苗,并比较了各自诱发的细胞免疫应答水平和对感染小鼠的保护力,
[Abstract]:Tuberculosis (Tuberculosis,TB) is a chronic infectious disease mainly caused by respiratory infection caused by Mycobacterium tuberculosis (Mycobacterium tuberculosis,MTB). According to WHO estimates, about one-third of the world's population is infected with MTB,. 10 million new cases and 3 million deaths occur every year. Poverty, population growth, increased floating population, increased drug-resistant MTB and the AIDS epidemic make the TB problem worse. China is one of the 22 countries with high burden of TB in the world. The number of people infected with MTB has reached 400 million and the number of patients with infectious TB has reached 2 million. BCG (BCG) is widely used to prevent TB, but its effect is not satisfactory. The study suggests that BCG can prevent and reduce severe TB, in children, but the preventive effect on adult TB varies from 0 to 80%. The causes of this phenomenon are the loss of protective antigen due to the variation of BCG strains, the genetic or nutritional differences among populations and the influence of environmental factors. In view of the deficiency of BCG itself and the severity of the current TB epidemic, the research on the application of new vaccines to the prevention of TB has become a hot research topic at home and abroad. ESAT6 and CFP10 in MTB early culture filtrate protein (CFP) are important protective antigens. In this study, subunit vaccines, gene vaccines and recombinant Mycobacterium smegmatis (mycobacterium smegmatis,M.S) vaccines expressing ESAT6 and CFP10 proteins were constructed successively, and the cellular immune response levels and the protective effects on infected mice were compared.
【学位授予单位】：第四军医大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R392
本文编号：2375840
[Abstract]:Tuberculosis (Tuberculosis,TB) is a chronic infectious disease mainly caused by respiratory infection caused by Mycobacterium tuberculosis (Mycobacterium tuberculosis,MTB). According to WHO estimates, about one-third of the world's population is infected with MTB,. 10 million new cases and 3 million deaths occur every year. Poverty, population growth, increased floating population, increased drug-resistant MTB and the AIDS epidemic make the TB problem worse. China is one of the 22 countries with high burden of TB in the world. The number of people infected with MTB has reached 400 million and the number of patients with infectious TB has reached 2 million. BCG (BCG) is widely used to prevent TB, but its effect is not satisfactory. The study suggests that BCG can prevent and reduce severe TB, in children, but the preventive effect on adult TB varies from 0 to 80%. The causes of this phenomenon are the loss of protective antigen due to the variation of BCG strains, the genetic or nutritional differences among populations and the influence of environmental factors. In view of the deficiency of BCG itself and the severity of the current TB epidemic, the research on the application of new vaccines to the prevention of TB has become a hot research topic at home and abroad. ESAT6 and CFP10 in MTB early culture filtrate protein (CFP) are important protective antigens. In this study, subunit vaccines, gene vaccines and recombinant Mycobacterium smegmatis (mycobacterium smegmatis,M.S) vaccines expressing ESAT6 and CFP10 proteins were constructed successively, and the cellular immune response levels and the protective effects on infected mice were compared.
【学位授予单位】：第四军医大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R392
【引证文献】
相关期刊论文 前1条
1 谢婷;李文桂;;结核分枝杆菌CFP10疫苗研究进展[J];中国病原生物学杂志;2007年03期
相关博士学位论文 前1条
1 简文;屋尘螨抗原Der p2重组耻垢分枝杆菌口服疫苗的制备及其实验研究[D];第四军医大学;2008年
相关硕士学位论文 前1条
1 谢婷;结核分枝杆菌重组Bb-ESAT-6-MPT64疫苗保护力及其免疫机制研究[D];重庆医科大学;2008年
，本文编号：2375840
本文链接：https://www.wllwen.com/yixuelunwen/binglixuelunwen/2375840.html
最近更新
教材专著
