HBx和HCV核心蛋白在HepG2细胞中协同增强环氧化酶-2的表达

发布时间：2018-12-14 06:59

【摘要】：原发性肝细胞性肝癌(HCC)是最常见的原发恶性肿瘤之一，死亡率极高，位居肿瘤发病率第五，占肿瘤死亡率的第四位。HCC的发生、发展是一个长期的、多因素、多步骤以及涉及多种基因及细胞功能改变的累积过程。在引发HCC的众多因素中，80％与乙型肝炎病毒(HBV)或／和丙型肝炎病毒(HCV)的持续感染及引起的肝硬化有关，由于HBV与HCV有相同的传播途径，，因此二者的混和感染比较常见，且临床流行病学调查结果显示，HBV和HCV混和感染可显著增加暴发性肝炎、肝硬化及患HCC的危险性，但目前对其机理仍然不完全清楚。环氧化酶-2(Cyclooxygenase-2，COX-2)的过表达与结肠癌、胃癌、肺癌、乳腺癌、胰腺癌和食道癌的发生均有关。对HCC临床标本的检测结果显示COX-2主要在高分化HCC和癌旁组织中表达较强，表达阳性率可达到70％以上。在肝癌细胞系中表达COX-2或加入前列腺素E_2(PGE_2)可促进肝癌细胞生长及增强肿瘤细胞的侵袭性，可抑制COX-2表达的非甾类抗炎药(nonsteroidal anti-inflammatory drugs，NSAIDs)可
[Abstract]:Primary hepatocellular carcinoma (HCC) is one of the most common primary malignant tumors, with a high mortality rate, ranking fifth in the incidence of cancer, occupying the fourth place in tumor mortality. The development of HCC is a long-term, multi-factor. Multiple steps and cumulative processes involving multiple gene and cell function changes. Of the many factors that cause HCC, 80% are related to the persistent infection of hepatitis B virus (HBV) or / and hepatitis C virus (HCV) and cirrhosis caused by HBV, because HBV and HCV have the same transmission route. The results of clinical epidemiological investigation showed that HBV and HCV mixed infection could significantly increase the risk of fulminant hepatitis, cirrhosis and HCC, but the mechanism was still unclear. The overexpression of cyclooxygenase-2 (Cyclooxygenase-2,COX-2) was associated with the occurrence of colon cancer, gastric cancer, lung cancer, breast cancer, pancreatic cancer and esophageal cancer. The results of clinical specimens of HCC showed that the expression of COX-2 was strong in highly differentiated HCC and paracancerous tissues, and the positive rate of COX-2 was more than 70%. The expression of COX-2 or the addition of prostaglandin E _ 2 (PGE_2) in hepatoma cell lines can promote the growth of hepatoma cells and enhance the invasiveness of tumor cells, and inhibit the expression of non-steroidal anti-inflammatory drugs (nonsteroidal anti-inflammatory drugs,NSAIDs), which can inhibit the expression of COX-2.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373;R735.7

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