肝细粒棘球蚴动物模型的建立及骨桥蛋白在外囊壁中的表达

发布时间：2018-12-16 23:16

【摘要】： 目的:建立肝包虫动物模型并观察包虫囊壁情况,研究骨桥蛋白在小鼠肝细粒棘球蚴囊壁周围的分布特征及表达。方法:选择同窝同种小鼠180只,随机分为免疫正常组及免疫抑制组,每组各90只,一组小鼠制作非特异性免疫抑制模型。将以上免疫正常及免疫抑制组按随机的方法又分别分为1、2、3组,每组30只,分别采用门静脉穿刺注射、开腹直视下肝脏穿刺注射及经皮肝脏穿刺注射细粒棘球蚴(Echinococcus granulosus,E.g)原头节混悬液的方法,制备肝E. g动物模型,免疫正常组作HE染色观察,免疫组化观察小鼠肝细粒棘球蚴外囊壁中骨桥蛋白(Osteopontin ,OPN)的表达与分布。结果:免疫正常组小鼠肝脏E. g感染率依据接种方法不同分别为93.1%、80.2%及59.3% ,免疫抑制组感染率分别为93.3%、79.3%及56.4%,两组无明显差异(P0.05)。HE切片染色显示外囊与周围组织具有明确界线,不存在渐变的过程, 83%肝细粒棘球蚴外囊壁外侧有不同程度OPN表达,且分布与囊壁外侧炎性细胞分布一致,在外囊层仅有少量OPN表达(P0.01)。结论:门静脉穿刺、开腹肝穿及经皮肝穿均可制备继发性肝脏E. g动物模型,且免疫正常组与抑制组感染率、包囊直径及包囊个数无明显差别,其中门静脉穿刺注射法较其他两种方法接种感染率高,包囊个数多,为理想的肝E. g动物模型制备方法,同时在动物模型上证实了早期肝细粒棘球蚴外囊为肉芽肿反应结果, OPN主要分布在肝细粒棘球蚴壁囊外侧炎性细胞中,并在外囊的形成过程中起重要作用。
[Abstract]:Aim: to establish an animal model of hepatic hydatid and to study the distribution and expression of osteopontin around the cyst wall of hepatic hydatid in mice. Methods: 180 homologous mice of the same nest were randomly divided into normal immune group and immunosuppressive group with 90 mice in each group. A non-specific immunosuppressive model was made in one group of mice. The above immune normal and immunosuppressive groups were randomly divided into 3 groups with 30 rats in each group. Each group was injected with portal vein puncture, liver puncture under open vision and percutaneous hepatic injection of (Echinococcus granulosus, (Echinococcus granulosus). E.G) the animal model of liver Eg was established by the suspension of proto-cephalic ganglion. The expression and distribution of osteopontin (Osteopontin, OPN) in the wall of hepatic hydatid of mice were observed by HE staining and immunohistochemistry. Results: according to the different inoculation methods, the liver Eg infection rate of the immunized normal mice was 93.2% and 59.3%, respectively. The infection rate of the immunosuppressive group was 93.3% and 56.4%, respectively, and that of the immunosuppressive group was 79.3% and 56.4%, respectively. There was no significant difference between the two groups (P0.05). HE staining showed that there was a clear boundary between the outer capsule and the surrounding tissues, and there was no gradual change. 83% of the patients had different degree of OPN expression in the outer wall of the epicystic wall of Echinococcus granulosus. The distribution was consistent with the distribution of inflammatory cells on the lateral wall of the capsule. There was only a small amount of OPN expression in the outer capsule (P0.01). Conclusion: portal vein puncture, open liver puncture and percutaneous liver puncture can make the animal model of secondary liver Eg, and there is no significant difference in infection rate, cyst diameter and number of cyst between immune normal group and inhibition group. Among them, the portal vein puncture injection method has higher infection rate and more cyst than the other two methods. It is an ideal method for the preparation of liver Eg animal model. At the same time, the results of granulomatous reaction of the early hepatic hydatid cyst were confirmed in the animal model. OPN mainly distributes in the inflammatory cells of the lateral wall of the hepatic hydatid wall and plays an important role in the formation of the outer capsule.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332

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