大鼠延髓巨细胞网状核与脑神经运动核的纤维联系

发布时间：2018-12-20 22:29

【摘要】： 位于脑干网状结构抑制区的巨细胞网状核(NRG)是一个功能复杂的核团，其无论是对躯体运动，还是对内脏活动都有影响。NRG对躯体运动的抑制性中枢调控作用，在神经系统电生理学及形态学研究中已得到充分证明，但该核团与内脏运动的联系尚缺乏全面、系统的资料。本实验拟从形态学上对巨细胞网状核的功能联系加以补充说明。目的 1．观察大鼠巨细胞网状核与脑神经运动核的直接纤维联系。 2．比较两种神经示踪剂WGA—HRP和DiI的标记效果。方法 1．用微量注射法将碳素墨水注入一侧巨细胞网状核，进行尼氏染色，观察NRG的定位准确性。 2．将WGA—HRP注入一侧NRG，采用TMB—ST法成色，光学显微镜下观察注射部位是否准确及标记情况。 3．将DiI注入一侧NRG，取材，荧光显微镜下观察注射部位及标记情况。结果 1．一般躯体运动核：动眼神经核、滑车神经核双侧可见逆行标记细胞及顺行神经纤维终末，逆行标记细胞同侧多于对侧；展神经核未见标记；舌下神经核双侧也看到逆行标记细胞及神经纤维终末。 2．一般内脏运动核：下泌涎核、迷走神经背核双侧均见少量逆行标记细胞及顺行神经纤维终末；上泌涎核未见标记。 3．特殊内脏运动核：面神经核、疑核、三叉神经运动核双侧均发现逆行标记细胞及顺行纤维末梢。 4．WGA—HRP和DiI标记的结果分布相似，不同的是，，DiI在下泌涎核未显示出标记，且在其他核团标记的细胞数目没有WGA—HRP标记的多。结论 1．大鼠巨细胞网状核与绝大多数脑神经运动核有纤维联系，且投射方向为双向。 2．DiI在成年动物活体脑中的标记效果不及WGA—HRP理想。
[Abstract]:(NRG) in the reticular nucleus of giant cells located in the inhibitory region of the reticular structure of the brain stem is a complex nuclear mass, which affects both the somatic movement and the visceral activity. The inhibitory central regulation of NRG on the somatic movement. It has been fully proved in the electrophysiology and morphology of nervous system, but the connection between the nucleus and visceral movement is still lack of comprehensive and systematic data. In this study, the functional connections of the reticular nucleus of giant cells were elucidated morphologically. Objective 1. To observe the direct fibrous connection between the reticular nucleus of giant cells and the motor nucleus of the brain. 2. The labeling effects of WGA-HRP and DiI were compared. Methods 1. Carbon ink was injected into one side of giant cell reticular nucleus by microinjection, and the localization accuracy of NRG was observed. 2. 2. The injection of WGA-HRP into one side of NRG, was colored by TMB-ST method, and the accuracy and labeling of the injection site were observed under optical microscope. 3. DiI was injected into one side of NRG, and the injection site and labeling were observed under fluorescence microscope. Results 1.General somatomotor nucleus: retrograde labeled cells and anterograde nerve fiber terminals were found on both sides of oculomotor nucleus, trochlear nucleus, ipsilateral retrograde labeled cells were more than contralateral, abductor nucleus was not labeled. Retrograde labeled cells and nerve fiber terminals were also seen on both sides of hypoglossal nucleus. 2. In general visceral motor nucleus, a few retrograde labeled cells and antegrade nerve terminals were found in the lower salivary nucleus and the dorsal nucleus of vagus, but no labeling was found in the upper salivary nucleus. 3. Special visceral motor nuclei: retrograde labeled cells and antegrade fibrous endings were found in the facial nucleus, the nucleus suspect and the motor nucleus of the trigeminal nerve. The results of 4.WGA-HRP and DiI labeling were similar, the difference was that DiI showed no markers in the lower salivary nucleus, and the number of cells labeled in other nuclei was not more than that labeled by WGA-HRP. Conclusion 1. The reticular nucleus of giant cells in rats has fibrous connections with most of the motor nuclei of the brain, and the projection direction is bidirectional. 2. The labeling effect of 2.DiI in the living brain of adult animals was not as good as that of WGA-HRP.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R322

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