人正常子宫内膜MC、VEGF、TSP表达与毛细血管超微结构研究

发布时间：2018-12-28 07:22

【摘要】：研究目的:1.利用免疫组织化学和图像分析技术定位正常子宫内膜上MC、VEGF和TSP的表达,分析其在增殖期、分泌期及绝经后子宫内膜的表达情况和分布特点的变化;2.利用透射电镜技术观察三期内膜中毛细血管的超微结构特征,尤其是它在基底层、功能层的变化情况。材料和方法:1.取人正常子宫增生期内膜组织(n=15),分泌期内膜组织(n=15),绝经期内膜组织(n=10),进行常规HE染色;应用类胰蛋白酶、VEGF Ab-7和TSPAb-4单克隆抗体,借助免疫组织化学SP法及图像分析系统,对正常子宫内膜的MC和血管生长因子进行研究;2.取人正常子宫增生期、分泌期、绝经期内膜组织各三例,利用透射电镜技术进行观察。结果:1、类胰蛋白酶定位于MC胞浆和脱颗粒中,对基底层MC进行单位面积阳性细胞计数:在分泌早期和绝经期减少,差别有统计学意义(5.20±0.94,1.32±0.39,P0.05);2、VEGF和TSP定位于子宫内膜腺上皮细胞、血管内皮细胞和间质细胞胞浆,二者在月经周期中表达强度以阳性单位PU值表示,在组间有统计学意义(P0.05);3、子宫内膜毛细血管超微结构显示:EC有丰富的胞质突,多伸向游离面,胞浆中见线粒体和粗面内质网聚集在一起,其结构在月经周期中未见明显变化。内膜功能层胞质突细小,主要伸向管腔游离面,与周细胞、间质细胞较少发生细胞-细胞接触;而基底层的胞质突起增多,有的质突粗大,末端出现分枝,与周细胞、间质细胞的细胞-细胞接触多发生。绝经期EC胞膜较平整,胞质突明显减少。结论:1、MC在增生期和分泌中晚期较多,分泌早期减少,绝经期中显著减少;2、VEGF和TSP的表达在育龄妇女增生期和分泌期子宫内膜中较绝经期妇女子宫内膜显著增强;3、子宫内膜血管的周期性生长是一个复杂过程,受VEGF、TSP和MC等多种因素的共同调控;4、子宫内膜基底层内皮细胞与周细胞、间质细胞的细胞间接触增多,可能参与内膜的周期性生长。
[Abstract]:Objective: 1. Immunohistochemical and image analysis techniques were used to locate the expression of MC,VEGF and TSP in normal endometrium, and to analyze the changes of expression and distribution of MC,VEGF and TSP in proliferative, secretory and postmenopausal endometrium. 2. The ultrastructural characteristics of the capillary in the third stage of the intima were observed by transmission electron microscopy (TEM), especially its changes in the basal layer and the functional layer. Materials and methods: 1. The normal endometrial tissue (nl15), secretory endometrial tissue (nm15) and menopausal endometrial tissue (nm10) were taken for routine HE staining. Using monoclonal antibodies of trypsin, VEGF Ab-7 and TSPAb-4, MC and vascular growth factor in normal endometrium were studied by immunohistochemical SP method and image analysis system. 2. Three cases of normal uterine hyperplasia, three cases of secretory phase and three cases of menopausal endometrium were collected and observed by transmission electron microscopy (TEM). Results: 1, trypsin was located in the cytoplasm and degranulation of MC. The number of positive cells per unit area of MC in basal layer was decreased in the early stage of secretion and menopause (5.20 卤0.94 卤1.32 卤0.39 P 0.05). 2VEGFand TSP were located in the cytoplasm of endometrial glandular epithelial cells, vascular endothelial cells and interstitial cells. 3. The ultrastructure of endometrial capillary showed that EC had abundant cytoplasmic processes, mostly extended to the free surface, mitochondria and rough endoplasmic reticulum gathered together in the cytoplasm, and its structure did not change obviously during the menstrual cycle. The endoplasmic processes of the intimal functional layer were small, mainly extended to the free surface of the lumen, and had little cell-cell contact with the pericytes and the interstitial cells. However, the number of cytoplasmic processes in the basal layer increased, some of them were coarse, branched at the end, and contact with pericytes and interstitial cells-cells occurred more frequently. In menopausal period, EC membrane was flat and cytoplasmic process was decreased. Conclusion: (1) the expression of TSP and TSP in proliferative and secretory endometrium was significantly higher than that in postmenopausal endometrium, and the expression of VEGFand TSP was significantly increased in proliferative and secretory endometrium, and decreased in early secretory phase and postmenopausal period, and the expression of VEGFand TSP in endometrium of postmenopausal women was higher than that of postmenopausal women. 3. The cyclical growth of endometrial blood vessels is a complex process, which is regulated by many factors such as VEGF,TSP and MC. 4. The intercellular contact between endothelial cells and pericytes and interstitial cells in the basal layer of endometrium may be involved in the periodic growth of endometrium.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R329

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