SD大鼠子宫内膜异位症模型的建立和评价

发布时间：2019-01-07 22:38

【摘要】： 目的：研究SD大鼠子宫内膜异位症模型建立，及己烯雌酚在大鼠子宫内膜异位症模型中的运用，并对模型作出评价。研究方法：将64只大鼠随意分4组建立模型。A组通过观察阴道脱落细胞学测动情周期2周，选动情期造模。B、C、D组适应性喂养4天后分别造模。B组造模前24小时给予己烯雌酚0.2mg／只灌胃促情。C组造模后第10天给予己烯雌酚0.02mg／kg／d灌胃，连续5d。D组造模前24小时予己烯雌酚0.2mg／只灌胃促情，造模后10天给予己烯雌酚0.02mg／kg／d，连续灌胃5d。建模时记录子宫形态直径，并取样做组织学观察。建模后4周第二次剖腹，观察移植物的形态颜色，，测量移植物的体积，并取移植物做组织学观察。结果：与动情期造模相比，术前给予己烯雌酚促情可以使大鼠的子宫在造模时处于充盈积水状态；从成模率上，D组大鼠(术前己烯雌酚促情同时在建模后给予了低剂量己烯雌酚促内膜生长)和其他组比有显著差异，说明己烯雌酚在此模型形成中可能其到一定的辅助作用；观察模型的特点，典型的模型可见移植物形成透明、半透明囊肿，表面可见血管形成，有的可呈现淡褐色，有的模型可向肌层呈浸润性生长；对模型的评价根据模型的色泽、透明度、与周围的对比度引入典型度的概念，典型度与模型的面积、高度、体积均有一定的相关性，利用典型度与体积等指标对模型联合评价应该能更准确地反映模型的状况。
[Abstract]:Aim: to study the establishment of endometriosis model in SD rats and the application of diethylstilbestrol in endometriosis model of rats, and to evaluate the model. Methods: 64 rats were randomly divided into 4 groups, A group was observed vaginal exfoliation cytology to measure estrus cycle for 2 weeks, and estrous period was selected to make model. Group D was fed with diethylstilbestrol (0.2mg/) 24 hours before modeling, and group C was given diethylstilbestrol 0.02mg/kg/d (0.02mg/kg/d) for 10 days. In 5d.D group, diethylstilbestrol (0.2mg/) was given intragastrically 24 hours before modeling, and diethylstilbestrol 0.02 mg / kg / d for 5 days. The uterine shape diameter was recorded and the histological observation was made. After 4 weeks of modeling, the second laparotomy was performed to observe the shape and color of the grafts, to measure the volume of the grafts, and to take the grafts for histological observation. Results: compared with the oestrous stage, the preoperation administration of diethylstilbestrol could make the rat uterus be filled with hydrops. There were significant differences in the rate of model formation between group D and other groups, indicating that diethylstilbestrol might play an auxiliary role in the formation of the model. The characteristics of the model were observed. The typical model showed transparent grafts, translucent cysts, angiogenesis on the surface, some of which were light brown, and some models showed infiltrative growth to the myometrium. The evaluation of the model is based on the color and transparency of the model, and introduces the concept of typicality with the contrast around the model. The model has a certain correlation with the area, height and volume of the model. The joint evaluation of the model with the index of canonical degree and volume should more accurately reflect the status of the model.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332;R711.71

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