Kruppel样因子6基因对巨噬细胞中胆固醇蓄积的影响

发布时间：2019-01-08 20:27

【摘要】：背景:研究表明,Kruppel样因子6与巨噬细胞极化密切相关。但是,关于Kruppel样因子6在巨噬泡沫细胞形成中的作用尚不清楚。目的:观察Kruppel样因子6过表达对氧化低密度脂蛋白刺激下的小鼠巨噬细胞(RAW264.7)胆固醇蓄积及对ATP结合盒转运蛋白A1表达的影响。方法:取Raw264.7巨噬细胞株分别转染慢病毒空载体和重组载体p CDH-KLF6,作为对照组和Kruppel样因子6组,另取稳定感染慢病毒空载体的Raw264.7巨噬细胞株和稳定感染重组载体p CDH-KLF6的Raw264.7巨噬细胞株均加入50 mg/L氧化低密度脂蛋白共孵育48 h后,得到氧化低密度脂蛋白组和Kruppel样因子6+氧化低密度脂蛋白组。结果与结论:与对照组相比,氧化低密度脂蛋白组巨噬细胞内总胆固醇和胆固醇酯表达水平显著增加(P0.05)。与氧化低密度脂蛋白组巨噬细胞相比,Kruppel样因子6+氧化低密度脂蛋白组细胞内总胆固醇和胆固醇酯表达水平明显下降,胆固醇酯/总胆固醇明显降低(P0.05)。而未加入氧化低密度脂蛋白处理的2组细胞内脂质表达水平少,且对照组与Kruppel样因子6组相比,细胞内脂质表达水平差异无显著性意义。提示Kruppel样因子6可能通过促进ATP结合盒转运蛋白A1的表达,抑制氧化低密度脂蛋白诱导的RAW264.7巨噬细胞胆固醇蓄积。
[Abstract]:Background: Kruppel-like factor 6 is closely related to macrophage polarization. However, the role of Kruppel-like factor 6 in macrophage foam formation is unclear. Aim: to investigate the effects of overexpression of Kruppel like factor 6 on cholesterol accumulation in mouse macrophages (RAW264.7) stimulated by oxidized low density lipoprotein (LDL) and on the expression of ATP binding cassette transporter A1 (ATP cassette transporter A1). Methods: Raw264.7 macrophages were transfected into lentivirus empty vector and recombinant vector p CDH-KLF6, as control group and Kruppel like factor 6 group respectively. In addition, the Raw264.7 macrophages infected with lentivirus empty vector and the Raw264.7 macrophages infected with recombinant vector p CDH-KLF6 were incubated with 50 mg/L oxidized low density lipoprotein for 48 h. Oxidized low density lipoprotein group and Kruppel like factor 6 oxidized low density lipoprotein group were obtained. Results and conclusion: compared with the control group, the expression of total cholesterol and cholesterol ester in macrophages of oxidized low density lipoprotein group was significantly increased (P0.05). Compared with oxidized low density lipoprotein group, the levels of total cholesterol and cholesterol ester in Kruppel like factor 6 oxidized low density lipoprotein group were significantly lower than those in oxidized low density lipoprotein group (P0.05). However, there was no significant difference in intracellular lipid expression between the control group and the Kruppel like factor 6 group in the two groups without the treatment of oxidized low density lipoprotein (OLDL). It is suggested that Kruppel like factor 6 may inhibit the accumulation of cholesterol in RAW264.7 macrophages induced by oxidized low density lipoprotein by promoting the expression of ATP binding cassette transporter A1.
【作者单位】：上海交通大学医学院附属第三人民医院心电图室;
【基金】：国家青年科学基金项目(81200206) 上海市卫生局课题(2010073)~~
【分类号】：R392

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