不同新生隐球菌菌株对人脐静脉内皮细胞损伤的初步研究

发布时间：2019-01-10 07:41

【摘要】：目的：探讨不同新生隐球菌菌株与人脐静脉内皮细胞(HUVEC)共孵后，新生隐球菌对HUVEC的抑制率和凋亡率的差异，为进一步的蛋白质组学筛选最大差异的菌株。方法：通过透射电子显微镜观察新生隐球菌侵入和损伤HUVEC的过程以及作用后细胞的形态学变化；并分别在不同时相点用CCK—8试剂盒检测细胞抑制率的变化；用流式细胞仪检测两者相互作用后细胞凋亡率的差异。结果和结论：通过透射电子显微镜观察到三种新生隐球菌菌株均有出芽状态，造成细胞不同程度的损伤。CCK—8试剂盒的检测结果表明，共孵30分钟时，三者的抑制率比较无统计学意义，共孵时间分别为1小时，，2小时，3小时，4小时时，荚膜缺陷株和白化株对内皮细胞的抑制率均高于野生株，5小时时，三者的抑制率在统计学上无明显差异。流式细胞仪的检测结果与其基本一致。
[Abstract]:Aim: to investigate the difference of inhibition rate and apoptosis rate of Cryptococcus neoformans (Cryptococcus neoformans) on HUVEC after co-incubation with human umbilical vein endothelial cells (HUVEC). Methods: the process of Cryptococcus neoformans invasion and injury of HUVEC and the morphological changes of cells were observed by transmission electron microscope, and the inhibition rate of Cryptococcus neoformans was detected by CCK-8 kit at different time points. Flow cytometry was used to detect the difference of apoptosis rate between the two groups. Results and conclusion: three Cryptococcus neoformans strains were observed to have budding state by transmission electron microscope, resulting in different degree of cell damage. The results of CCK-8 kit showed that after 30 minutes incubation, The inhibition rates of the three strains were not statistically significant. The inhibitory rates of the capsule defect strain and albino strain on endothelial cells were higher than that of the wild strain at 1 h, 2 h, 3 h and 4 h, respectively, and at 5 h, the inhibition rate of the three strains on endothelial cells was higher than that of the wild strain. There was no statistical difference in the inhibition rate among the three groups. The results of flow cytometry were in good agreement with them.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R379

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