树突状细胞介导母胎免疫耐受机制中作用的研究

发布时间：2019-02-16 22:12

【摘要】：第一章正常月经周期妇女、正常妊娠和早期自然流产妇女外周血中树突状细胞亚群的研究目的:研究树突状细胞(dendritic cell, DC)亚群髓样DC(myeloid dendritic cell, MDC)和浆细胞样DC(plasmacytoid dendritic cell, PDC)在正常月经周期妇女、正常妊娠和早期自然流产妇女外周血中的变化,探讨DC亚群介导母胎免疫耐受机制中的作用。方法:选择正常月经周期妇女30例,分别在每个妇女月经周期卵泡期和黄体期采集外周血。正常妊娠妇女30例,分别在每个妊娠妇女早期(8～10周)、中期(20～22周)、晚期(36～38周)妊娠和正常分娩后42天采集外周血,并在分娩时采集脐血,正常未孕妇女为对照组。早期自然流产妇女30例采集外周血,正常早期妊娠妇女为对照组。从外周血中分离单个核细胞进行四色免疫荧光标记,然后应用流式细胞术,检测各组外周血单个核细胞中MDC和PDC的百分率及MDC/PDC比率。应用放射免疫法分别检测卵泡期、黄体期、早期自然流产和正常早期妊娠妇女外周血中雌二醇和孕酮的水平。结果:正常月经周期黄体期外周血中MDC的百分率和MDC/PDC比率明显低于卵泡期(P0.01),PDC的百分率与卵泡期无显著性差异(P0.05)。正常月经
[Abstract]:Chapter 1 study on dendritic cell subsets in peripheral blood of women with normal menstrual cycle, normal pregnancy and early spontaneous abortion objective: to study dendritic cell (dendritic cell, DC) subsets myeloid DC (myeloid dendritic cell, MDC) and plasmacyte-like DC (plasmacytoid dendritic cell, PDC) in peripheral blood of women with normal menstrual cycle, normal pregnancy and early spontaneous abortion. To explore the role of DC subsets in maternal and fetal immune tolerance. Methods: peripheral blood samples were collected from 30 normal menstrual cycle women in follicular phase and luteal phase respectively. The peripheral blood was collected from 30 normal pregnant women at the early stage (8 weeks), the second trimester (20 weeks), the third trimester (36 ~ 38 weeks) and 42 days after normal delivery, and the umbilical cord blood was collected at the time of delivery. Normal non-pregnant women served as control group. Peripheral blood was collected from 30 women with early spontaneous abortion and control group with normal early pregnancy. The percentage of MDC and PDC and the ratio of MDC/PDC in peripheral blood mononuclear cells were detected by flow cytometry. The levels of estradiol and progesterone in peripheral blood of women in follicular phase luteal phase early spontaneous abortion and normal early pregnancy were detected by radioimmunoassay. Results: the percentage of MDC and the ratio of MDC/PDC in luteal phase of normal menstrual cycle were significantly lower than those in follicular phase (P0.01), PDC and follicular phase P 0.05). Normal menstruation
【学位授予单位】：暨南大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R392

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