利用层粘连蛋白扩增罗曼鹤鸡骨髓间充质干细胞

发布时间：2019-02-20 18:34

【摘要】：背景:鸡骨髓间充质干细胞是胚胎发育学、免疫学和肿瘤学研究重要的细胞模型,但如何大规模扩增并使其保持良好的未分化潜能是鸡骨髓间充质干细胞应用中亟待解决的难题。目的:建立扩增鸡骨髓间充质干细胞的层粘连蛋白培养体系。方法:将体外分离得到的鸡骨髓间充质干细胞分别接种在包被层粘连蛋白培养皿和传统二维培养皿上。经过体外扩增,比较两种培养体系下,鸡骨髓间充质干细胞的形态特征、表面标志物、扩增性能和成脂分化潜能。结果与结论:层粘连蛋白培养体系和常规培养体系中骨髓间充质干细胞的形态学特征和表面标志物表达没有显著差异,但层粘连蛋白培养体系获得的骨髓间充质干细胞增殖能力和分化潜能都明显优于传统培养体系。可见层粘连蛋白培养体系能快速地扩增出大量增殖能力强和未分化性能良好的鸡骨髓间充质干细胞,为鸡骨髓间充质干细胞的进一步深入研究和应用提供生物学基础。
[Abstract]:Background: chicken bone marrow mesenchymal stem cells are important cell models for embryonic development, immunology and oncology. However, how to amplify and maintain good undifferentiated potential on a large scale is an urgent problem in the application of chicken bone marrow mesenchymal stem cells. Objective: to establish a laminin culture system for the expansion of chicken bone marrow mesenchymal stem cells. Methods: chicken bone marrow mesenchymal stem cells isolated in vitro were inoculated on coated laminin culture dish and traditional two-dimensional culture dish respectively. The morphological characteristics, surface markers, amplification properties and adipogenic potential of chicken bone marrow mesenchymal stem cells were compared in vitro. Results and conclusion: there was no significant difference in morphology and expression of surface markers of bone marrow mesenchymal stem cells between laminin culture system and conventional culture system. However, the proliferation and differentiation potential of bone marrow mesenchymal stem cells obtained by laminin culture system were obviously superior to those of traditional culture system. It can be seen that laminin culture system can rapidly expand a large number of chicken bone marrow mesenchymal stem cells with strong proliferative and undifferentiated ability, which provides a biological basis for further research and application of chicken bone marrow mesenchymal stem cells.
【作者单位】：哈尔滨医科大学附属第一医院;大连医科大学公共卫生学院;
【基金】：国家自然科学基金(81102160,81273038)~~
【分类号】：R329

【参考文献】