螺钉压迫法致上颈髓损伤的动物模型建立及评价

发布时间：2019-02-22 17:47

【摘要】：[目的]建立并评估螺钉压迫法致上颈髓损伤的动物模型,为研究上颈髓损伤的机制及临床治疗提供实验基础。[方法]健康成年雄性大耳兔36只,随机分为模型组和假手术组,模型组采用螺钉压迫C2段的脊髓建立上颈髓损伤的动物模型,假手术组仅暴露C2椎板后即刻缝合,术后1、3、7、14 d进行行为学Tarlov评分,原位末端脱氧核糖核酸转移酶介导的脱氧尿苷三磷酸(dUTP)标记测定法(Tunel法)检测脊髓神经细胞的凋亡水平,逆转录-聚合酶链式反应(RT-PCR)检测caspase-3 mRNA的表达。[结果]两组在不同时间点的Tarlov评分、Tunel阳性细胞数、caspase-3 mRNA的相对表达量比较均具有统计学差异(P0.05),且后二者观察指标的表达在时间上具有相关性。[结论]本研究建立的上颈髓损伤动物模型,能够基本反映上颈髓损伤的病理生理过程,并具有较好的操作性及重复性,可用于上颈髓损伤的研究。
[Abstract]:[objective] to establish and evaluate the animal model of upper cervical spinal cord injury induced by screw compression in order to provide experimental basis for studying the mechanism and clinical treatment of upper cervical spinal cord injury. [methods] Thirty-six healthy adult male rabbits were randomly divided into two groups: model group and sham operation group. The model group used screw compression of C2 segment spinal cord to establish the animal model of upper cervical spinal cord injury. The sham operation group only exposed C2 lamina and sutured immediately after operation. The behavioral Tarlov scores were measured at day 7 and 14, and the level of apoptosis in spinal cord neurons was detected by in situ terminal deoxyribonucleic acid transferase-mediated deoxyuridine triphosphate (dUTP) labeling (Tunel method). The expression of caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). [results] the Tarlov score, the number of Tunel positive cells and the relative expression of caspase-3 mRNA were significantly different between the two groups at different time points (P0.05), and the expression of the latter two indexes was correlated in time. [conclusion] the animal model of upper cervical spinal cord injury established in this study can basically reflect the pathophysiological process of upper cervical spinal cord injury, and has good maneuverability and repeatability, so it can be used in the study of upper cervical spinal cord injury.
【作者单位】：湖北中医药大学;广州军区武汉总医院骨科;
【基金】：全军医学科学技术研究“十一五”计划攻关课题(编号:08G031)
【分类号】：R-332;R651.2

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