PAGE-4、LAGE-1和NXF-2三种CT抗原单克隆抗体的制备及其表达分布的研究

发布时间：2019-02-25 12:37

【摘要】：目前恶性肿瘤仍然是威胁人类生命健康的首要疾病。随着对睾丸/肿瘤相关抗原(CT抗原)研究的不断深入,其在肿瘤基础研究以及诊断和治疗中的地位也日趋重视。然而关于CT抗原的研究目前更多的是停留在DNA及mRNA水平,对于其在蛋白质水平上的认识大多是限于对其免疫原性的分析,这种滞后状态的主要原因是缺乏特异性的抗CT抗原的单克隆抗体。为了在蛋白质水平上研究PAGE-4、LAGE-1和NXF-2三种CT抗原,本文从制备高特异性、高敏感性单克隆抗体入手,结合在DNA及mRNA方面的有关资料及实验方法,研究PAGE-4、LAGE-1和NXF-2在正常组织和肿瘤组织中的表达分布规律以及与肿瘤分期分级的关系。应用淋巴细胞杂交瘤技术,制备和鉴定了PAGE-4、LAGE-1和NXF-2三种CT抗原的单克隆抗体,并筛选出特异性强及敏感性高并识别天然抗原的相应单克隆抗体,LX-PAGE4.2、LX-LAGE1.9和LX-NXF2,免疫组织化学染色最佳工作浓度分别为0.5μg/ml、2.5μg/ml、5.0μg/ml。以
[Abstract]:At present, malignant tumor is still the primary disease threatening human life and health. With the development of testicular / tumor-associated antigen (CT), more and more attention has been paid to its role in the basic research, diagnosis and treatment of cancer. However, most of the studies on CT antigens remain at the level of DNA and mRNA, and most of their knowledge on protein level is limited to the analysis of their immunogenicity. The main reason for this lagging state is the lack of specific monoclonal antibodies against CT antigens. In order to study three kinds of CT antigens of PAGE-4,LAGE-1 and NXF-2 at the protein level, this paper starts with the preparation of highly specific and highly sensitive monoclonal antibodies, combines the relevant data and experimental methods in DNA and mRNA, and studies PAGE-4,. The expression and distribution of LAGE-1 and NXF-2 in normal tissues and tumor tissues and their relationship with tumor staging and grading. The monoclonal antibodies against PAGE-4,LAGE-1 and NXF-2 antigens were prepared and identified by lymphocyte hybridoma technique, and the corresponding monoclonal antibodies with strong specificity and high sensitivity and recognition of natural antigens, LX-PAGE4.2, were screened. The best working concentration of LX-LAGE1.9 and LX-NXF2, immunohistochemical staining was 0.5 渭 g / ml ~ (2.5 渭 g / ml) and 5.0 渭 g / ml ~ (-1), respectively. With
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392

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