人类巨细胞病毒UL144、UL146和UL147基因多态性研究
发布时间：2019-03-02 19:54
【摘要】：目的 小儿先天畸形是影响我国出生人口素质的重要因素,宫内感染是引起新生儿先天畸形的重要因素之一,而人类巨细胞病毒(Human Cytomagelovirus,HCMV)则在引起先天宫内感染的微生物中居于首位。综合国内外研究结果知,活产新生儿的先天性HCMV感染率约为0.3%-2.3%,平均为1%。先天感染儿中,有症状或明显改变的占5%,有轻微症状或非典型症状改变的占5%。其余90%HCMV感染表现为亚临床或慢性感染,这其中5-15%HCMV感染儿多在生后2年内发生神经性耳聋或不同程度的神经精神运动障碍。HCMV感染引起的先天疾病或畸形主要包括:黄疸性肝炎、胆道闭锁、先天性巨结肠等肝胆消化系统疾病或畸形;小头畸形、智力发育迟缓等神经系统畸形等。部分HCMV先天感染儿也可表现为无症状带毒者。最近研究证明不同的HCMV分离株可在体内表现出不同的细胞嗜性。我们以往的研究资料也显示,神经系统畸形患儿HCMV分离株比肝胆消化系统畸形患儿HCMV分离株的首次病毒分离周期短(分别为平均9.5天和19.5天);且HCMV血清抗体与HCMV病毒多肽的反应性在不同系统畸形患儿之间存在差异。 目前,HCMV感染的致病机理尚不清楚,大多数学者认为与病毒的自身结构,特别是与病毒的毒力、组织细胞嗜性及其逃避机体免疫能力密切相关的病毒基因差异,可能是决定HCMV先天感染致病多样性的重要因素。这些病毒基因的多态性可能是HCMV致畸、致病的根本原因。因此,针对HCMV不同分离株中与病毒的致病性密切相关的病毒基因的各项研究已然成为目前国际巨细胞病毒研究最活跃的研究领域。1998年,Wirgart等用DNA测序的方法研究了HCMV基因组中与病毒毒力相关的直接早期启动子和增强子、与病毒复制相关的DNA聚合酶及与病毒免疫原性相关的gB蛋白基因等基因序列在不同HCMV临床分离株之间的差别,虽证实上述差
[Abstract]:Objective congenital malformation in children is an important factor affecting the quality of birth population in China. Intrauterine infection is one of the important factors causing congenital malformation in newborns. Human cytomegalovirus (Human Cytomagelovirus,) is one of the important factors. HCMV) is the leading cause of infection in the prehistoric palace. According to the results of domestic and foreign studies, the congenital HCMV infection rate of live births is about 0.3% ~ 2.3%, with an average of 1%. Of children with congenital infections, 5% had symptoms or significant changes, and 5% had mild or atypical symptoms. The remaining 90%HCMV infections were subclinical or chronic. The congenital diseases or malformations caused by 5-15%HCMV infection mainly include icterohepatitis, biliary atresia, choledochal atresia and choledochal atresia. Congenital Hirschsprung's disease or deformity of hepatobiliary digestive system; Microcephaly, mental retardation and other neurological abnormalities. Some children with congenital HCMV infection may also be asymptomatic carriers. Recent studies have shown that different HCMV isolates exhibit different cellular tropism in vivo. Our previous studies also showed that the first virus isolation cycle of HCMV isolates from children with nervous system malformation was shorter than that of HCMV isolates from children with hepatobiliary and digestive system malformations (mean 9.5days and 19.5days, respectively). Moreover, the reactivity of HCMV serum antibody to HCMV virus polypeptide was different in children with different systemic malformations. At present, the pathogenesis of HCMV infection is not clear. Most scholars believe that the viral gene difference is closely related to the structure of the virus, especially the virulence of the virus, the tropism of tissues and the ability to evade the immunity of the body. It may be an important factor to determine the diversity of congenital infection of HCMV. The polymorphism of these virus genes may be the root cause of teratogenesis and pathogenesis of HCMV. Therefore, various studies on the viral genes closely related to the pathogenicity of viruses in different strains of HCMV have become the most active research field in the field of cytomegalovirus research in the world. Wirgart and others used DNA sequencing method to study the direct early promoter and enhancer related to virus virulence in HCMV genome. Differences of DNA polymerase associated with viral replication and gB protein gene sequences associated with viral immunogenicity among different HCMV clinical isolates, although these differences were confirmed.
【学位授予单位】：中国医科大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R373
本文编号：2433410
[Abstract]:Objective congenital malformation in children is an important factor affecting the quality of birth population in China. Intrauterine infection is one of the important factors causing congenital malformation in newborns. Human cytomegalovirus (Human Cytomagelovirus,) is one of the important factors. HCMV) is the leading cause of infection in the prehistoric palace. According to the results of domestic and foreign studies, the congenital HCMV infection rate of live births is about 0.3% ~ 2.3%, with an average of 1%. Of children with congenital infections, 5% had symptoms or significant changes, and 5% had mild or atypical symptoms. The remaining 90%HCMV infections were subclinical or chronic. The congenital diseases or malformations caused by 5-15%HCMV infection mainly include icterohepatitis, biliary atresia, choledochal atresia and choledochal atresia. Congenital Hirschsprung's disease or deformity of hepatobiliary digestive system; Microcephaly, mental retardation and other neurological abnormalities. Some children with congenital HCMV infection may also be asymptomatic carriers. Recent studies have shown that different HCMV isolates exhibit different cellular tropism in vivo. Our previous studies also showed that the first virus isolation cycle of HCMV isolates from children with nervous system malformation was shorter than that of HCMV isolates from children with hepatobiliary and digestive system malformations (mean 9.5days and 19.5days, respectively). Moreover, the reactivity of HCMV serum antibody to HCMV virus polypeptide was different in children with different systemic malformations. At present, the pathogenesis of HCMV infection is not clear. Most scholars believe that the viral gene difference is closely related to the structure of the virus, especially the virulence of the virus, the tropism of tissues and the ability to evade the immunity of the body. It may be an important factor to determine the diversity of congenital infection of HCMV. The polymorphism of these virus genes may be the root cause of teratogenesis and pathogenesis of HCMV. Therefore, various studies on the viral genes closely related to the pathogenicity of viruses in different strains of HCMV have become the most active research field in the field of cytomegalovirus research in the world. Wirgart and others used DNA sequencing method to study the direct early promoter and enhancer related to virus virulence in HCMV genome. Differences of DNA polymerase associated with viral replication and gB protein gene sequences associated with viral immunogenicity among different HCMV clinical isolates, although these differences were confirmed.
【学位授予单位】：中国医科大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R373
【参考文献】
相关期刊论文 前4条
1 何蓉,刘兰青,吕绳敏,阮强;荧光定量PCR方法检测婴儿尿液中人类巨细胞病毒基因的含量[J];中华儿科杂志;2001年12期
2 阮强,卢颖,何蓉,吉耀华,齐莹,刘庆,陈淑荣,马艳萍;人巨细胞病毒UL136基因在临床低传代分离株中多态性分析[J];中华微生物学和免疫学杂志;2003年09期
3 齐莹,卢颖,马艳萍,阮强,何蓉,吉耀华,刘庆,陈淑荣;人巨细胞病毒UL138～142基因在临床低传代分离株中的多态性研究[J];中华微生物学和免疫学杂志;2004年03期
4 吉耀华,阮强,何蓉,齐莹,马艳萍,孙峥嵘,刘庆,陈淑荣,周艳丽;人巨细胞病毒UL149基因在临床低传代分离株中的多态性研究[J];中华微生物学和免疫学杂志;2004年12期
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