电脉冲介导的DNA疫苗骨骼肌内免疫接种的转基因长期表达和抗原递呈机理的研究

发布时间：2019-03-06 15:48

【摘要】： 电脉冲介导的DNA免疫(DNA+EP)可以明显增强DNA疫苗的免疫效果。但其中的作用机理不明确,我们希望从DNA+EP免疫转基因表达,和DNA+EP免疫后抗原递呈两个角度,讨论其中的具体过程和作用机理。我们发现,DNA+EP免疫对作用区域的骨骼肌会造成一定程度的损伤,骨骼肌的创伤随着离电极距离增加而减弱。在电极附近,电脉冲将骨骼肌的肌束膜和肌内膜击穿,释放出游离的肌细胞,这些肌细胞由于受到过度损伤而发生横纹肌溶解导致坏死/凋亡;在离电极较远的区域,电脉冲仅将肌束膜击穿,释放出游离的肌细胞,但并不会引起这些细胞坏死/凋亡。 DNA+EP免疫对骨骼肌的损伤将在局部区域引发较严重的炎症反应。DNA+EP作用2小时后,受损伤部位的骨骼肌中有大量的血球渗出,这些细胞多数是多形核细胞。到第3天,炎症浸润细胞中多
[Abstract]:Electric pulse-mediated immunization of (DNA EP) with DNA can significantly enhance the immune effect of DNA vaccine. However, the mechanism of action is not clear. We hope to discuss the specific process and mechanism of DNA EP immuno-transgene expression and antigen presentation after DNA EP immunization from two angles. We found that, DNA EP immunity can cause some damage to skeletal muscle in the acting area, and the injury of skeletal muscle decreases with increasing distance from electrode. Near the electrode, electrical pulses break through the muscle envelope and endomembrane of the skeletal muscle and release free muscle cells, which result in necrosis / apoptosis due to excessive injury of the striated muscle due to the dissolution of the rhabdomyolysis. In the region far away from the electrode, electrical pulses only break through the muscle bundle and release free muscle cells, but do not cause necrosis / apoptosis of these cells. The injury of skeletal muscle induced by DNA EP immunization will cause severe inflammation in the local area. After 2 hours of EP treatment, a large number of blood cells exudate from the injured skeletal muscle, and most of these cells are polymorphic cells. 2 hours after exposure to EP, a large number of blood cells exudate from the injured skeletal muscle, and most of these cells are polymorphic cells. By day 3, there were more inflammatory infiltrating cells
【学位授予单位】：上海交通大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R392

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