碘缺乏或碘过量大鼠海马和小脑形态学改变的实验研究

发布时间：2019-04-02 15:19

【摘要】：目的观察以不同浓度碘离子(KIO_3)水喂养Wistar成年大鼠3个月和6个月后,其大脑海马神经元和神经胶质细胞、大脑皮质内内锥体细胞层的锥体细胞以及小脑的形态学改变,探讨碘缺乏和碘过量对成年大鼠大脑皮质、海马及小脑形态及功能的影响。方法断乳一个月的Wistar大鼠(60日龄),雌雄各半,随机分为6组:①低碘组(LI组);②适碘组(NI组);③5倍碘组(5HI组);④10倍碘组(10HI组);⑤50倍碘组(50HI组)。⑥100倍碘组(100HI组)。LI组动物饲以低碘地区的粮食(平均碘含量小于50μg/kg),饮用去离子水;其它5组饲以正常鼠料,(平均碘含量300μg/kg),NI组饮用自来水(碘含量为5μg/L),5HI、10HI、50HI、100HI组分别饮用含不同浓度的KIO_3的自来水。按每日进食量和饮水量计算各组动物每日摄碘量估计如下:1μg/L(LI),6.15μg/L(NI),30.75μg/L(5HI),61.5μg/L(10HI),307.5μg/L(50HI),615μg/L(100HI)。用以上方法喂养3个月和6个月后,观察大脑皮质、海马以及小脑的形态学改变(焦油紫染色、免疫组化染色和镀银染色),并对海马NSE阳性神经元和GFAP阳性星形胶质细胞的体积密度(Vv)、面数密度(N_A)和细胞质的平均灰度值进行形态计量学分析,计数大脑皮质内内锥体细胞层锥体细胞的基底树突数。分析小脑分子层、颗粒层和蒲肯野细胞层等各层的面积比,以及蒲肯野细胞层内蒲氏细胞胞体中尼氏体与其胞体的面积比。结果 1.实验3个月和6个月低碘组及各碘过量组与NI组比较,全脑绝对重量、相对重量无明显差异。2.实验3个月和6个月各碘过量组与NI组比较,甲状腺相对重量无明显改变,而低碘组与同月份NI组比较,甲状腺相对重量明显增加(P0.01)。3.对海马CA_3区的研究结果:与同月份NI组比较,实验3个月
[Abstract]:Objective to observe the hippocampal neurons and glial cells in hippocampus of adult Wistar rats fed with different concentrations of iodine ion (KIO_3) for 3 months and 6 months. The morphological changes of pyramidal cells and cerebellum in the inner pyramidal layer of cerebral cortex were studied to explore the effects of iodine deficiency and excess iodine on the morphology and function of cerebral cortex, hippocampus and cerebellum in adult rats. Methods Wistar rats (60 days old) who were weaned for one month were randomly divided into 6 groups: 1 low iodine group (LI group), 2 normal iodine group (NI group), 35 times iodine group (5HI group), 410 times iodine group (10HI group). 6100 times iodine group (100HI group). LI group) were fed with deionized water in low iodine area (the average iodine content was less than 50 渭 g / kg),). The average iodine content was less than 50 渭 g / kg), in 50HI group. The other 5 groups were fed with normal mice (the average iodine content was 300 渭 g / kg), NI), drinking tap water (iodine content was 5 渭 g / L), 5 HI, 10 HI, 50 HI, 100 HI groups), and drinking tap water with different concentrations of KIO_3 respectively in the 100 HI group (the iodine content was 5 渭 g / L), 5 HI, 10 HI, 50 HI). The daily iodine intake was estimated as follows: 1 渭 g / L (LI), 6.15 渭 g / L (NI), 30.75 渭 g / L (5HI), 61.5 渭 g / L (10HI), 307.5 渭 g / L (50HI), 615 渭 g / L (100HI). After 3 and 6 months of feeding, the morphological changes of cerebral cortex, hippocampus and cerebellum (tar violet staining, immunohistochemical staining and silver-plated staining) were observed. The volume density of NSE-positive neurons and GFAP-positive astrocytes in hippocampus, (Vv), area numerical density (Na), and the mean gray value of cytoplasm were analyzed by morphometry. The number of basal dendrites of pyramidal cells in the inner pyramidal layer of the cerebral cortex was counted. The area ratios of cerebellum molecular layer, granular layer and Purkinje cell layer were analyzed, and the area ratio of Nissl body to body of Purkinje cell in Purkinje cell layer was analyzed. Outcome 1. There was no significant difference in the absolute weight and relative weight of the whole brain between the iodine deficiency group and the iodine excess group after 3 months and 6 months compared with the NI group. 2. Compared with NI group, the thyroid relative weight of iodine excess group did not change significantly after 3 months and 6 months of experiment, but the thyroid relative weight of low iodine group was significantly higher than that of NI group in the same month (P0.01). Results of the study on the hippocampal CA_ 3 region: compared with the NI group in the same month, the experiment lasted for 3 months.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R361

【相似文献】