乙型肝炎病毒多聚酶末端蛋白的克
发布时间：2019-04-12 13:37
【摘要】：全球HBV的感染者已超过3亿，，人类迫切需要研究新型而有效治疗乙肝的手段。乙型肝炎在临床上的表现千变万化，可导致患者机体严重损害，甚至危及生命，给临床医生的诊断和治疗带来极大的挑战。为了准确恰当的评估乙肝患者的病情，必须依赖相关的实验室检测和辅助检查，主要包括HBV血清标志物检测、肝脏酶类生化检测、肝组织病理学等传统实验室检查方法以及B超、CT、MRI等彩像学检查及胃镜等器械检查，随着分子生物学和医学免疫学的迅速发展以及对此疾病的更加深刻地认识，现在已存在建立更加敏感和早期的乙型病毒性肝炎的诊断和治疗方法的可能。 乙型肝炎病毒多聚酶在整个复制过程中起着关键作用，包括正链DNA的修补、ε信号识别、蛋白引发、转位、逆转录、DNA依赖的DNA聚合酶活性和RnaseH等活性。有学者推测急慢性乙肝感染中TP可能分别起不同作用。对TP功能分子机制的阐明，有可能提示决定HBV急性感染转归的关键因素。定性和定量地检测TP在肝细胞中的表达以
[Abstract]:There are more than 300 million HBV infected people all over the world. It is urgent to study new and effective methods to treat hepatitis B. The clinical manifestation of hepatitis B is ever-changing, which can lead to serious damage to the patient body, even life-threatening, and bring great challenges to the diagnosis and treatment of clinicians. In order to accurately and properly evaluate the condition of hepatitis B patients, we must rely on the relevant laboratory tests and auxiliary tests, mainly including the detection of HBV serum markers, liver enzymes biochemical test, With the rapid development of molecular biology and medical immunology and the deeper understanding of the disease, traditional laboratory examination methods such as liver histopathology, B-ultrasound, CT,MRI and other imaging examinations, and gastroscopy and other instruments, have developed rapidly with the rapid development of molecular biology and medical immunology. It is now possible to establish more sensitive and early methods for diagnosis and treatment of hepatitis B. Hepatitis B virus polymerase plays a key role in the whole replication process, including positive chain DNA repair, 蔚 signal recognition, protein initiation, translocation, reverse transcription, DNA-dependent DNA polymerase activity and RnaseH activity. Some scholars speculate that TP may play a different role in acute and chronic hepatitis B infection. The elucidation of the functional molecular mechanism of TP may suggest the key factors that determine the outcome of acute HBV infection. The expression of TP in hepatocytes was detected qualitatively and quantitatively.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392
本文编号：2457065
[Abstract]:There are more than 300 million HBV infected people all over the world. It is urgent to study new and effective methods to treat hepatitis B. The clinical manifestation of hepatitis B is ever-changing, which can lead to serious damage to the patient body, even life-threatening, and bring great challenges to the diagnosis and treatment of clinicians. In order to accurately and properly evaluate the condition of hepatitis B patients, we must rely on the relevant laboratory tests and auxiliary tests, mainly including the detection of HBV serum markers, liver enzymes biochemical test, With the rapid development of molecular biology and medical immunology and the deeper understanding of the disease, traditional laboratory examination methods such as liver histopathology, B-ultrasound, CT,MRI and other imaging examinations, and gastroscopy and other instruments, have developed rapidly with the rapid development of molecular biology and medical immunology. It is now possible to establish more sensitive and early methods for diagnosis and treatment of hepatitis B. Hepatitis B virus polymerase plays a key role in the whole replication process, including positive chain DNA repair, 蔚 signal recognition, protein initiation, translocation, reverse transcription, DNA-dependent DNA polymerase activity and RnaseH activity. Some scholars speculate that TP may play a different role in acute and chronic hepatitis B infection. The elucidation of the functional molecular mechanism of TP may suggest the key factors that determine the outcome of acute HBV infection. The expression of TP in hepatocytes was detected qualitatively and quantitatively.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392
【参考文献】
相关期刊论文 前6条
1 周建衡,洪振丰;慢性肝炎检测指标的研究进展[J];福建中医学院学报;2005年02期
2 骆欢;詹雨林;赵志新;;慢性乙型肝炎临床与病理的相关性研究进展[J];临床肝胆病杂志;2005年06期
3 陈长征,黄华,龚健,夏其昌,的伯良,王应睐;大肠杆菌His_6融合表达载体及其表达产物的一步纯化[J];生物化学与生物物理学报;1996年05期
4 吕娇健,孙慧伶,丁友法,张洪梅;乙型肝炎病毒DNA含量与血清标志物的关系[J];上海医学检验杂志;2001年06期
5 邱英华,沈益,王玉海,徐贤秀,董雪吟,张洪祖;Cecropin-X发酵过程中工程菌质粒稳定性的研究[J];微生物学报;2004年03期
6 单晶,丁惠国;乙型肝炎病毒基因型的临床意义[J];中西医结合肝病杂志;2003年05期
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