兔颈总动脉破裂粥样硬化斑块模型的建立

发布时间：2019-04-17 20:31

【摘要】：目的建立负压吸引激发兔颈总动脉粥样硬化斑块破裂,建立破裂斑块模型。方法将30只健康雄性新西兰白兔随机分为3组,分别为对照组(n=10)、高脂饲养组(n=10)和球囊损伤+高脂饲养组(n=10)。饲养8周后,彩色多普勒超声测量颈总动脉内径、内-中膜厚度(IMT)及颈动脉血流速度等,评估动脉IMT与动脉粥样硬化程度的关系。采用"腔外负压"法诱导斑块破裂并处死动物,观察诱发斑块破裂成功率,在肉眼及光镜下观察颈动脉内膜形态特征。结果球囊损伤+高脂饲养组、高脂饲养组的颈总动脉血流速度显著快于对照组,IMT显著厚于对照组,颈总动脉内径显著短于对照组;球囊损伤+高脂饲养组的颈总动脉内径显著短于高脂饲养组,IMT显著厚于高脂饲养组,颈总动脉血流速度显著快于高脂饲养组(P0.05)。球囊损伤+高脂饲养组可见平滑肌细胞增生,脂质、炎症细胞、泡沫细胞浸润,符合不稳定性斑块特征,有8例诱发斑块破裂,1例死亡;高脂饲养组可见内膜下脂质沉积,炎症细胞浸润,但内皮细胞完整,内弹力板排列整齐,1例诱发斑块破裂,无动物死亡;对照组内皮细胞完整,中层平滑肌细胞排列整齐,无一例诱发斑块破裂,无动物死亡。结论本实验建立动脉粥样硬化斑块破裂模型成功率高,动物死亡率低,实验操作简便易行,为冠心病的生理、病理及药物学效应方面研究提供了可靠的实验平台。
[Abstract]:Objective to establish a rabbit carotid atherosclerotic plaque rupture model by negative pressure suction. Methods Thirty healthy male New Zealand white rabbits were randomly divided into three groups: control group (n = 10), high fat feeding group (n = 10) and balloon injury group (n = 10). After 8 weeks of feeding, the internal diameter of common carotid artery, intima-media thickness (IMT) and blood flow velocity of carotid artery were measured by color Doppler ultrasound. The relationship between IMT and the degree of atherosclerosis was evaluated. "Extraluminal negative pressure" was used to induce plaque rupture and the animals were killed to observe the successful rate of plaque rupture, and to observe the morphological characteristics of carotid intima under naked eyes and light microscope. Results the blood flow velocity of the common carotid artery in the high fat feeding group was significantly faster than that in the control group, the IMT was significantly thicker than that in the control group, and the internal diameter of the common carotid artery was significantly shorter than that in the control group. The internal diameter of common carotid artery in the high fat feeding group was significantly shorter than that in the high fat feeding group, and the IMT was significantly thicker than the high fat feeding group. The blood flow velocity of the common carotid artery was significantly faster than that of the high fat feeding group (P0.05). Smooth muscle cell proliferation, lipid, inflammatory cells and foam cell infiltration were observed in the high fat feeding group with balloon injury, which was consistent with the characteristics of unstable plaques. 8 cases induced plaque rupture and 1 case died. In the high fat feeding group, subintimal lipid deposition and infiltration of inflammatory cells were observed, but endothelial cells were intact, internal elastic plates were arranged neatly, one case induced plaque rupture, and no animal death was found. In the control group, endothelial cells were intact, smooth muscle cells in the middle layer were arranged neatly, and no plaque rupture or animal death was induced in the control group. Conclusion the experimental model of atherosclerotic plaque rupture has a high success rate, low animal mortality, simple and easy operation, and provides a reliable experimental platform for the study of physiological, pathological and pharmacological effects of coronary heart disease.
【作者单位】：郑州大学附属郑州中心医院心内科;
【基金】：河南省郑州市科技攻关计划郑州市博士基金项目资助(2010-010021)
【分类号】：R-332

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