锌对辣椒素致痛模型小鼠脊髓c-fos表达和动物行为学的影响

发布时间：2019-04-25 13:49

【摘要】：目的研究锌对辣椒素致痛模型小鼠脊髓c-fos表达和动物行为学的影响。方法 48只C57/BL6小鼠随机分为4组,采用足底注射辣椒素(0.5%,5μl)制备神经病理性疼痛(NPP)小鼠模型:对照组(Con)给予适锌饲料(锌30mg/kg/d)喂养2周后,右后足底注射溶剂(吐温80、酒精和生理盐水混合液);适锌喂养组(N-NPP)给予适锌饲料(锌30mg/kg/d)喂养2周后足底注射辣椒素;低锌喂养组(L-NPP)给予低锌饲料(锌0.85mg/kg/d)喂养2周后注射辣椒素;高锌喂养组(H-NPP)给予硫酸锌水溶液(227mg/L/d)喂养2周后注射辣椒素。应用原子吸收光谱技术、动物行为学观察、免疫组织化学和图像分析技术检测注射辣椒素后7d锌对脊髓cfos表达和动物行为学的影响。结果高锌喂养能显著增加血清和脊髓中锌的含量,使脊髓c-fos表达下调,机械痛阈增高,痛敏减弱(0.01);而低锌喂养加重血清和脊髓的缺锌状态,脊髓c-fos表达上调,机械痛阈降低,痛敏增强(0.01)。结论锌能抑制辣椒素致痛模型小鼠脊髓c-fos的表达,降低痛敏。
[Abstract]:Objective to study the effect of zinc on the expression of c-fos and animal behavior in spinal cord of capsaicin-induced pain model mice. Methods 48 C57/BL6 mice were randomly divided into 4 groups. The (NPP) model of neuropathic pain was made by injection of capsaicin (0.5%, 5 渭 l) into the foot. (Con) in control group was fed with zinc-appropriate diet (Zn-30mg/kg/d) for 2 weeks, and the control group was fed with zinc-appropriate diet (Zn-30mg/kg/d) for 2 weeks. Right posterior plantar injection of solvent (Tween 80, mixture of alcohol and saline); The rats in the N-NPP group were fed with zinc-appropriate diet (Zn-30mg/kg/d) for 2 weeks, and the rats in the low-Zn group (L-NPP) were fed with low-Zn diet (Zn-0.85mg/kg/d) for 2 weeks, and the capsaicin was injected into the foot after 2 weeks of feeding with zinc-appropriate diet (Zn-0.85mg/kg/d). High zinc feeding group (H-NPP) was fed with zinc sulfate aqueous solution (227mg/L/d) for 2 weeks and then capsaicin was injected. The effects of zinc on the expression of cfos in spinal cord and animal behavior were detected by atomic absorption spectroscopy (AAS), animal behavior observation, immunohistochemistry and image analysis techniques 7 days after injection of capsaicin. Results the content of zinc in serum and spinal cord was significantly increased, the expression of c-fos in spinal cord was decreased, the threshold of mechanical pain was increased, and the sensitivity of pain was weakened. Low zinc diet aggravated zinc deficiency in serum and spinal cord, increased the expression of c-fos in spinal cord, decreased the mechanical pain threshold and increased pain sensitivity. Conclusion Zinc can inhibit the expression of c-fos and decrease pain sensitivity in spinal cord of capsaicin-induced pain model mice.
【作者单位】：辽宁医学院组织胚胎学教研室;辽宁省锦州市中心医院肾内科;
【基金】：辽宁省科技厅基金(20111118) 辽宁省大学生创新训练计划项目(201210160007) 辽宁医学院奥鸿大学生创新基金(2011D17) 辽宁医学院横向基(LYHX2013025,2013024) 奥鸿博泽研究生科新基金(2012009)
【分类号】：R363

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