番茄红素对兔动脉粥样硬化形成和内皮细胞氧化损伤的影响

发布时间：2019-05-10 14:58

【摘要】：目前认为动脉粥样硬化疾病表现了一种高氧化应激状态，活性氧(reactiveoxygen species，ROS)诱导的氧化应激在动脉粥样硬化疾病的发病中有重要作用。在动脉粥样硬化形成和发展的病理过程，活性氧不仅氧化修饰低密度脂蛋白，促进泡沫细胞形成，而且活性氧介导的脂质过氧化还可以破坏血管内皮细胞，导致其功能障碍，从而促进动脉粥样硬化发生发展。在冠心病的发病过程中从初发脂纹到斑块损伤发展，内皮功能受损具有十分重要的意义。血管内皮细胞功能障碍是动脉粥样硬化的早期病理改变，而脂质过氧化则可能为内皮功能受损的始动环节之一。因此，抗氧化剂通过阻止氧化应激损伤对动脉粥样硬化疾病的防治是有积极意义的。大量的流行病学资料显示，，番茄红素的摄入量与冠心病的危险性呈负相关，血浆低番茄红素有更高的急性心肌梗死和脑卒中的危险性。但其防治这些疾病的具体机制尚不清楚。番茄红素是很好的抗氧化剂，通过阻断或抑制氧化应激损伤可能是番茄红素抗动脉粥样硬化的形成的一条途径。我们试图通过动物实验和细胞实验研究探讨其抗动脉粥样硬化疾病的作用及机制。第一部分番茄红素对兔动脉粥样硬化形成的影响目的观察番茄红素对高脂喂饲所致新西兰家兔动脉粥样硬化形成和脂质过氧化损伤的影响。方法采用高脂喂饲模型，将30只新西兰家兔随机分为3组：对照组、高脂模型组和番茄红素组，分别给予普通饲料和高脂饲料喂养，番茄红素组另加用番茄红素(每兔每天每公斤体重给予6％番茄红素粉剂60mg)；于实验开始前1天和开始后第2、4、6、8周末取空腹血测定血清总胆固醇，甘油三酯，高密度脂蛋白胆固醇，血清超氧化物歧化酶活性，丙二醛含量，血清一氧化氮含量和一氧
[Abstract]:It is believed that atherosclerotic diseases exhibit a state of high oxidative stress. Oxidative stress induced by reactive oxygen species (reactiveoxygen species,ROS) plays an important role in the pathogenesis of atherosclerotic diseases. In the pathological process of atherosclerosis formation and development, reactive oxygen species (Ros) not only oxidize low density lipoprotein and promote foam cell formation, but also destroy vascular endothelial cells and lead to dysfunction of vascular endothelial cells mediated by reactive oxygen species (Ros). So as to promote the occurrence and development of atherosclerosis. The damage of endothelial function is of great significance in the pathogenesis of coronary heart disease (CAD), from the primary lipid lines to plaque injury. Vascular endothelial cell dysfunction is an early pathological change of atherosclerosis, and lipid peroxidation may be one of the initiation of endothelial dysfunction. Therefore, antioxidants are of positive significance in the prevention and treatment of atherosclerosis by preventing oxidative stress injury. A large number of epidemiological data show that the intake of lycopene is negatively correlated with the risk of coronary heart disease, plasma low lycopene has a higher risk of acute myocardial infarction and stroke. However, the specific mechanism of its prevention and treatment of these diseases is not clear. Lycopene is a good antioxidant. Blocking or inhibiting oxidative stress damage may be a way of lycopene anti-atherosclerosis formation. We tried to explore the effect and mechanism of anti-atherosclerosis through animal and cell experiments. Part one effect of lycopene on atherogenesis in rabbits objective to observe the effect of lycopene on atherosclerosis formation and lipid peroxidation injury induced by high fat feeding in New Zealand rabbits. Methods 30 New Zealand rabbits were randomly divided into three groups: control group, high fat model group and lycopene group. Lycopene group was treated with lycopene (6% lycopene powder 60mg per kilogram body weight per rabbit per day). Serum total cholesterol, triglyceride, high density lipoprotein cholesterol, serum SOD activity, malondialdehyde content, serum nitric oxide content and monooxia were measured 1 day before and 2, 4, 6 and 8 weeks after the beginning of the experiment.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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