不同品系小鼠感染小鼠肝炎病毒后IgG、IgM抗体变化及肝脏病毒检测

发布时间：2019-05-18 21:16

【摘要】： 小鼠肝炎病毒(MHV)是一种动物冠状病毒。采用MHV-A59标准毒株通过三种途径(腹腔、滴鼻、灌胃)对6个品系的实验小鼠(BALB/c、C57、nu/nu、NIH-Ⅲ、IL-4T、IL-10T)进行了病毒感染。通过检测小鼠感染后一个月内不同时段的抗体变化,发现小鼠在感染MHV后,血清转阳一般发生在感染后5～7天,3周左右特异性的IgG水平达到高峰。而特异性的IgM水平出现上升时间比IgG早,3天左右就开始升高,7天到达高峰,3周后开始下降。但不同品系小鼠由于遗传背景不同对MHV的易感性和耐受性存在明显差异。不同感染途径之间也存在着一定的差异,腹腔感染MHV的小鼠抗体转阳时间和IgG、IgM高峰水平出现的时间都较滴鼻和灌胃途径早。而滴鼻感染MHV后小鼠抗体转阳时间出现较晚,但其维持时间较长,所以认为MHV感染后产生免疫力的持久性在很大程度上取决于免疫途径的选择。用小鼠肝炎病毒A59株以滴鼻、灌胃、腹腔注射三种途径人工感染近交系小鼠(BALB/c小鼠、C57BL小鼠)、免疫缺陷小鼠(NIH-Ⅲ小鼠、BALB/c-nu/nu小鼠)、转基因小鼠(IL-4T鼠、IL-10T鼠),接种病毒后3、5、7、14、21、28d分别取各鼠的肝脏,提取肝脏总RNA,用RT-PCR方法检测小鼠肝炎病毒的感染情况。结果显示:腹腔注射病毒的小鼠在肝脏中能最早检测出病毒,其次是灌胃,滴鼻则最晚;另外,SPF小鼠及免疫缺陷小鼠感染后3d在肝脏中检测出病毒,而转基因小鼠则在感染后7d在肝脏中检测出病毒。
[Abstract]:Mouse hepatitis virus (MHV) is an animal coronavirus. Six strains of experimental mice (BALB/c,C57,nu/nu,NIH- III, IL-4T,IL-10T) were infected by MHV-A59 standard strain by three ways (abdominal cavity, nasal drip, intragastrically). By detecting the changes of antibody in different periods within one month after infection, it was found that after infection with MHV, the serum positive rate generally occurred at 5 to 7 days after infection, and the specific IgG level reached the peak at about 3 weeks after infection. The specific IgM level increased earlier than IgG, began to rise in about 3 days, reached the peak in 7 days, and began to decrease after 3 weeks. However, the susceptibility and tolerance of different strains of mice to MHV were significantly different due to different genetic background. There were also some differences among different routes of infection. The antibody conversion time and the peak level of IgG,IgM in mice infected with MHV were earlier than those by nasal infusion and intragastric administration. However, after nasal infection with MHV, the antibody positive time appeared late, but it lasted for a long time, so it is considered that the persistence of immunity after MHV infection depends to a large extent on the choice of immune pathway. Inbred mice (BALB/c mice, C57BL mice) and immunodeficient mice (NIH- III mice, BALB/c-nu/nu mice) were artificially infected with mouse hepatitis virus A59 strain by nasal drip, intragastric administration and intraabdominal injection. The liver of transgenic mice (IL-4T mice, IL-10T mice) were taken from the livers of each mouse at 3, 5, 7, 14, 21 and 28 days after vaccination, and the total RNA, was extracted to detect the infection of hepatitis virus in mice by RT-PCR method. The results showed that the virus could be detected in the liver of mice injected intravenously, followed by intragastric administration and nasal drip. In addition, the virus was detected in the liver of SPF mice and immunodeficient mice 3 days after infection, while the virus was detected in the liver of transgenic mice 7 days after infection.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

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