近日节律系统对药物依赖和学习记忆的影响
发布时间：2019-05-19 16:10
【摘要】：目的: 本文从药物依赖和学习记忆的角度,研究近日节律系统对高级神经活动的影响和作用。包括两部分:1、近日节律基因mPerl与吗啡依赖的关系,探讨近日节律基因mPerl在药物依赖过程中的作用和机制;2、不同光暗循环与学习记忆的关系,研究不同光暗循环对小鼠学习记忆的影响和机制。 方法: 实验一:近日节律基因mperl在小鼠吗啡依赖中的作用 动物选用BALB/C小鼠。设计并合成针对mPerl的“10-23”DNA核酶。以无DNA核酶活性的寡核苷酸对照,对同时吗啡皮下注射和DNA核酶脑室注射的小鼠以及先吗啡皮下注射后DNA核酶脑室注射的小鼠进行研究。通过条件性位置偏爱实验,检测小鼠对吗啡的药物依赖;以Western blot法,检测小鼠全脑mPERl的表达;同时,体外实验验证DNA核酶对mPerlmRNA的切割效率。 实验二:不同光暗循环对小鼠学习记忆的影响 动物选用ICR小鼠。小鼠在控制环境条件下,人工提供光线,3组的光暗循环分别为LD5/5、LD12/12和LD22/22,饲养6周。检测指标包括:小鼠的活动性、血浆皮质激素(放射免疫法)和学习记忆能力(Morris水迷宫)。 结果: 实验一:DNA核酶不但在体外能够切割mPerl mRNA,而且在体
[Abstract]:Objective: to study the effect and effect of circadian rhythm system on advanced nerve activity from the point of view of drug dependence and learning and memory. It includes two parts: 1. The relationship between circadian rhythm gene mPerl and morphine dependence, to explore the role and mechanism of circadian rhythm gene mPerl in the process of drug dependence; 2. The relationship between different light and dark cycles and learning and memory was studied, and the effect and mechanism of different light and dark cycles on learning and memory in mice were studied. Methods: experiment 1: the role of circadian rhythm gene mperl in morphine dependence in mice. BALB/C mice were selected. A "10 脳 23" DNA ribozyme for mPerl was designed and synthesized. Compared with oligodeoxynucleotides without DNA ribozyme activity, mice injected subcutaneously with morphine and intraventricular injection of DNA ribozyme and mice injected with morphine subcutaneous injection followed by intraventricular injection of DNA ribozyme were studied. The drug dependence of mice on morphine was detected by conditioned place preference test, the expression of mPERl in the whole brain of mice was detected by Western blot assay, and the cutting efficiency of DNA ribozyme on mPerlmRNA was verified in vitro. Experiment 2: effects of different light and dark cycles on learning and memory in mice ICR mice were selected. Under the control environment, the mice were artificially supplied with light, and the light and dark cycles of the three groups were fed with LD5/5,LD12/12 and LD22/22, for 6 weeks, respectively. The activity of mice, plasma corticosteroids (radioimmunity) and learning and memory ability (Morris water maze) were measured. Results: experiment 1: DNA ribozyme could not only cut mPerl mRNA, in vitro but also in vivo.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R33;R749.6
本文编号：2480865
[Abstract]:Objective: to study the effect and effect of circadian rhythm system on advanced nerve activity from the point of view of drug dependence and learning and memory. It includes two parts: 1. The relationship between circadian rhythm gene mPerl and morphine dependence, to explore the role and mechanism of circadian rhythm gene mPerl in the process of drug dependence; 2. The relationship between different light and dark cycles and learning and memory was studied, and the effect and mechanism of different light and dark cycles on learning and memory in mice were studied. Methods: experiment 1: the role of circadian rhythm gene mperl in morphine dependence in mice. BALB/C mice were selected. A "10 脳 23" DNA ribozyme for mPerl was designed and synthesized. Compared with oligodeoxynucleotides without DNA ribozyme activity, mice injected subcutaneously with morphine and intraventricular injection of DNA ribozyme and mice injected with morphine subcutaneous injection followed by intraventricular injection of DNA ribozyme were studied. The drug dependence of mice on morphine was detected by conditioned place preference test, the expression of mPERl in the whole brain of mice was detected by Western blot assay, and the cutting efficiency of DNA ribozyme on mPerlmRNA was verified in vitro. Experiment 2: effects of different light and dark cycles on learning and memory in mice ICR mice were selected. Under the control environment, the mice were artificially supplied with light, and the light and dark cycles of the three groups were fed with LD5/5,LD12/12 and LD22/22, for 6 weeks, respectively. The activity of mice, plasma corticosteroids (radioimmunity) and learning and memory ability (Morris water maze) were measured. Results: experiment 1: DNA ribozyme could not only cut mPerl mRNA, in vitro but also in vivo.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R33;R749.6
【引证文献】
相关硕士学位论文 前2条
1 史书;强制隔离戒毒所戒毒人员个别心理治疗研究[D];湘潭大学;2009年
2 堵吉;持续光照对雌性大鼠生殖内分泌节律的影响及滋阴补阳序贯中药干预作用的实验研究[D];南京中医药大学;2012年
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