门静脉回流大鼠胰肾联合移植模型的建立及ICOS单抗联合CTLA4Ig在大鼠胰腺移植中的作用研究

发布时间：2019-05-20 10:00

【摘要】： 目的：建立一个稳定、可靠、符合生理模式的胰肾联合移植模型。方法：以雄性健康近交系SD大鼠作为实验动物，予链脲佐菌素(streptozotocin，STZ)50mg／kg体重一次性尾静脉注射，建立糖尿病大鼠模型。注射后隔日测血糖及尿糖，血糖连续两次超过16.8mmol／L且尿糖反应强阳性者判断为糖尿病诱导成功，，作为移植受体使用。行整块胰肾十二指肠移植，采用供体腹主动脉和受体腹主动脉端侧吻合，供体门静脉和受体肠系膜静脉端侧吻合，供体肾静脉和受体肾静脉袖套式吻合，供体十二指肠与受体空肠侧侧吻合，供体输尿管膀胱瓣与受体膀胱吻合。结果：共正式实验30例，以术后血糖正常且存活5天以上为标准，成功率达到80％。供体手术时间为(50.90±3.13)min，受体手术时间为(73.03±3.85)min。术前以及术后第1，3，5天的血糖分别为(18.0±0.54)、(4.76±0.12)、(5.18±0.10)、(5.69±0.12)mmol／L。结论：我们通过这种方法建立的模型是稳定、可靠的，可用于胰肾联合移植基础方面的研究。
[Abstract]:Objective: to establish a stable, reliable and physiological model of simultaneous pancreas-kidney transplantation. Methods: the diabetic rat model was established by tail vein injection of streptozotocin (streptozotocin,STZ) 50mg/kg in male healthy inbred SD rats. Blood glucose and urine glucose were measured every other day after injection. the patients whose blood glucose exceeded 16.8mmol/L twice in a row and whose urine glucose reaction was strong positive were judged to be successful in inducing diabetes mellitus and were used as recipients of transplantation. End-to-side anastomosis of donor abdominal aortic and recipient ventral aortic, end-to-side anastomosis of donor portal vein and recipient mesenteric vein, cuff anastomosis of donor renal vein and recipient renal vein were performed. The donor duodenum was anastomosed with the recipient Jejunum, and the donor ureter bladder flap was anastomosed with the recipient bladder. Results: a total of 30 cases were tested formally. The success rate was 80% according to the standard of normal postoperative blood glucose and survival for more than 5 days. The donor operation time was (50.90 卤3.13) min, and the operation time was (73.03 卤3.85) min.. Before operation and on the 1st and 3rd day after operation, the blood glucose was (18.0 卤0.54), (4.76 卤0.12), (5.18 卤0.10), (5.69 卤0.12) mmol/L., respectively. Conclusion: the model established by this method is stable and reliable, and can be used in the study of the basis of simultaneous pancreas-kidney transplantation.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332;R657.5

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