低强度脉冲超声对人脂肪干细胞成骨诱导影响的实验研究

发布时间：2019-05-22 22:51

【摘要】：[目的]探讨低强度脉冲超声在人脂肪干细胞成骨诱导中的影响。[方法]取手术患者腹部及腰背部处脂肪,采用胶原酶消化离心贴壁法分离培养脂肪间充质干细胞(adipose derived stem cells,ADSCs),流式细胞仪检测细胞表面标记。第6代细胞分为四组:(1)阴性对照组:ADSCs于完全培养基中培养,不接受LIPUS剌激;(2)LIPUS组:ADSCs于完全培养基中培养,接受LIPUS刺激;(3)成骨诱导组:ADSCs于成骨诱导培养基中培养;(4)LIPUS联合成骨诱导组:ADSCs在成骨诱导培养基中培养,接受LIPUS刺激。[结果]ADSCs形态传至10代时仍无变化。流式细胞仪分析第6代hADSCs细胞表面特异性标记结果显示,CD105:99.61%,CD29:97.54%,CD45:0.26%,CD44:97.4%,CD14:2.51%。LIPUS+诱导组的碱性磷酸酶(ALP)表达明显高于其他组,而单用LIPUS,并不能促进成人脂肪干细胞成骨分化。[结论]LIPUS可以促进成骨诱导培养基培养下的成人脂肪干细胞的成骨分化。
[Abstract]:Objective to investigate the effect of low intensity pulse ultrasound on osteogenic induction of human adipose stem cells. [methods] Fat from abdomen and waist and back of surgical patients were isolated and cultured by collagenase digestion and centrifugal adherent method. Cell surface labeling was detected by flow cytometry. The 6th passage cells were divided into four groups: (1) negative control group: ADSCs was cultured in complete medium without LIPUS stimulation, (2) LIPUS group: ADSCs was cultured in complete medium and stimulated by LIPUS. (3) osteogenic induction group: ADSCs was cultured in osteogenic induction medium, (4) LIPUS combined with osteogenic induction group: ADSCs was cultured in osteogenic induction medium and stimulated by LIPUS. [results] there was no change in the morphology of ADSCs from 10 generations to 10 generations. The surface specific labeling of passage 6 hADSCs cells was analyzed by flow cytometry. The results showed that CD105:99.61%,CD29:97.54%,CD45:0.26%,CD44:97.4%, The expression of alkaline phosphatase (ALP) in CD14:2.51%.LIPUS induced group was significantly higher than that in other groups, but LIPUS, alone could not promote osteogenic differentiation of adult adipose stem cells. [conclusion] LIPUS can promote osteogenic differentiation of adult adipose stem cells cultured in osteogenic induction medium.
【作者单位】：江苏省无锡市人民医院骨科;
【基金】：无锡市医管中心重大协作项目(编号:YGZX1111)
【分类号】：R329.2

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