兼性胞内寄生菌HLK1～T对人宿主细胞的感染和持续性寄生的研究

发布时间：2019-06-14 16:17

【摘要】：目前,病原菌仍是人类健康最大的威胁之一,比如急性呼吸道感染、腹泻、肺结核等疾病都是由于病原菌的感染所致。而且,新的细菌感染性疾病的不断出现,以及大量致病菌的反复出现和耐药性的增加,对公众健康造成可怕的威胁。因此,对潜在病原菌的发现以及病原菌致病机理的研究一直是研究的热点之一。人红白血病细胞系K562细胞被广泛用于实验室的研究。当我们对K562细胞进行核型分析过程中,意外地发现K562细胞染色标本中有杆状细菌样的结构。为了排除该实验中相关实验试剂的环境细菌污染,我们严格控制了各种实验试剂和实验操作。然而,多次重复实验仍得到以上结果。因此,我们推论该细胞中可能存在一种细胞内生菌。将K562细胞、RPMI 1640完全培养基以及各种实验用试剂分别涂至基础血平板(5%绵羊血)上,置37℃孵箱中培养48～72小时,结果发现涂有K562细胞的平板上有菌落生成,而其它平板均无任何菌落形成。经多次实验证明,平板上形成的细菌集落确为K562细胞来源。只得注意的是,K562细胞能保持良好的生长状态和增殖能力,而且细胞培养基中无明显的细菌污染迹象。以上初步的实验结果提示,寄生在K562细胞内的细菌可能是一种新的细胞内生菌,因为目前已明确的能寄生在人类细胞内的胞内寄生菌在细胞内会大量繁殖,因此感染的细胞不能健康生长。由于该菌可能是一种新的胞内寄生菌,就有必要明确该菌在细菌分类中的地位,以及该菌的基本的生物学特性。第一部分:HLK1~T的分离和鉴定目的:对细菌的分类鉴定是了解其生物学功能和特性的基础。为明确HLK1~T的分类地位及其基本生物学特性,该研究从人红白血病细胞系562细胞中分离获得
[Abstract]:At present, the pathogenic bacteria are still one of the greatest threats to human health, such as acute respiratory tract infection, diarrhea, pulmonary tuberculosis and other diseases due to the infection of the pathogenic bacteria. Moreover, the emergence of new bacterial infectious diseases, as well as the repeated appearance and resistance of a large number of pathogenic bacteria, poses a terrible threat to public health. Therefore, the research on the discovery of the potential pathogenic bacteria and the pathogenesis of the pathogenic bacteria has been one of the hot spots of the study. Human erythroleukemia cell line K562 cells are widely used It was used in the research of the laboratory. In the process of the karyotype analysis of K562 cells, it was found that the K562 cells were stained with staining. The structure of the rod-shaped bacteria-like structure. In order to eliminate the environmental bacterial contamination of the relevant experimental reagents in the experiment, we have strictly controlled the various facts The test reagent and the experimental operation. However, the test is repeated many times. The above results are still available. Therefore, we can infer that the cells may be stored in the cell In a cell endophytic bacterium, K562 cells, RPMI 1640 full culture medium, and various experimental reagents are respectively coated on a basic blood plate (5% sheep blood), and incubated for 48-72 hours in a 37-degree-C cold box, and the results show that the plate coated with K562 cells has colony formation, while other plates None of the colonies were formed. The colony of bacteria formed on the plate was proved to be true by multiple experiments. The K562 cell is a source of cell. It is important to note that K562 cells can maintain good growth and proliferation, and the cell culture medium is not clear. The above preliminary experimental results suggest that the bacteria that are parasitic in the K562 cells may be a new endogenetic strain, as the intracellular parasitic bacteria that can be parasitically in the human cell can be propagated in a large amount in the cell, thus infecting The cell cannot grow well. As the bacteria may be a new intracellular parasitic, it is necessary to identify the status of the bacterium in the bacterial classification, and the bacteria. Basic biological characteristics. Part 1: Separation and identification of HLK1-T Objective: The classification and identification of bacteria is the basis of understanding the biological functions and characteristics of the bacteria. In order to clarify the classification status of HLK1-T and its basic life
【学位授予单位】：浙江大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R378

【共引文献】