机械通气致兔肺损伤后TNF-α、IL-8时序性表达规律的研究

发布时间：2019-06-18 12:25

【摘要】：研究目的及背景．肿瘤坏死因子-α(TNF-α)和白介素-8(IL-8)在创伤性急性肺损伤、创伤性休克致急性肺损伤均有表达，但迄今为止，过度机械通气致肺损伤后TNF-α和IL-8的表达时序性规律尚未见报道。本研究主要是对兔进行过度机械通气后TNF-α和IL-8的表达规律进行研究，并且对机械通气性肺损伤的分子机制进行了进一步的了解，也为机械通气致肺损伤的分子病理诊断、法医学鉴定提供理论依据。方法：将健康新西兰兔随机分为空白组、对照组及实验组。实验组按照损伤后处死时间分为0小时、0.5小时、1小时、1.5小时、3小时、6小时、12小时、18小时和24小时9个亚组。应用组织芯片、免疫组化及免疫蛋白印迹方法对兔肺组织中的TNF-α、IL-8的表达进行研究。结果： (1)空白组及对照组各时间点肺组织内均有低水平的TNF-α、IL-8表达，它们之间的差异无统计学意义。(2)机械通气后TNF-α、IL-8表达明显增加，较对照组比较有统计学意义。(3)机械性通气致肺损伤后：TNF-α 0.5小时上升，1.5小时达高峰，，2小时下降，6小时后接近对照组水平。IL-8 1.5小时开始升高，6小时仍继续上升，12小时开始下降，24小时达对照组。结论：机械通气致肺损伤后可诱导TNF-α、IL-8在肺组织内表达增加，TNF-α、IL-8的表达改变的时序性规律可望作为一种客观指标用于法医学机械性肺损伤的早期损伤经历时间的推断。
[Abstract]:Objective and background. Tumor necrosis factor-伪 (TNF- 伪) and IL-8 (IL-8) have been expressed in both traumatic acute lung injury and traumatic shock induced acute lung injury, but up to now, the timing of TNF- 伪 and IL-8 expression after lung injury induced by hypermechanical ventilation has not been reported. The purpose of this study was to study the expression of TNF- 伪 and IL-8 after hypermechanical ventilation in rabbits, and to further understand the molecular mechanism of mechanical ventilation induced lung injury, and to provide theoretical basis for molecular pathological diagnosis and forensic identification of lung injury induced by mechanical ventilation. Methods: healthy New Zealand rabbits were randomly divided into blank group, control group and experimental group. According to the time of death after injury, the experimental group was divided into 9 subgroup: 0 hours, 0.5 hours, 1 hour, 1.5 hours, 3 hours, 6 hours, 12 hours, 18 hours and 24 hours. The expression of TNF- 伪 and IL-8 in rabbit lung tissue was studied by tissue microarray, immunohistochemistry and immunoblotting. Results: (1) there were low levels of TNF- 伪 and IL-8 expression in lung tissue of blank group and control group at each time point, and there was no significant difference between them. (2) the expression of TNF- 伪 and IL-8 increased significantly after mechanical ventilation, which was significantly higher than that of control group. (3) after lung injury induced by mechanical ventilation, TNF- 伪 increased 0. 5 hours. Il-8 reached the peak at 1.5 hours, decreased at 2 hours, and approached the level of the control group after 6 hours. Il-8 began to rise at 1.5 hours, continued to rise at 6 hours, began to decrease at 12 hours, and reached the control group at 24 hours. Conclusion: after lung injury induced by mechanical ventilation, the expression of TNF- 伪 and IL-8 in lung tissue is increased. The timing of the expression of TNF- 伪 and IL-8 is expected to be used as an objective index to infer the early injury time of mechanical lung injury in forensic medicine.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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