3种方法对发热伴血小板减少综合征检测结果比较

发布时间：2019-07-01 18:24

【摘要】：目的采用实时荧光PCR法、酶联免疫吸附法及细胞培养分离病毒株的方法,对2013年临床疑似发热伴血小板减少综合征患者急性期血进行检测比较,寻求快速、准确的鉴定新布尼亚病毒感染的方法。方法对180例临床疑似病例的急性期血液首先采用实时荧光PCR法检测,筛选出新布尼亚病毒核酸阳性的样本,同时将阳性样本采用酶联免疫吸附的方法检测抗体及采用VERO-E6细胞培养的方法分离病毒株。结果 180份样本经实时荧光PCR法检测,阳性率为42.78%(77/180Ct值≤35曲线形态与阳性对照一致呈S型的),ELISA方法检测抗体阳性率为44.16%(34/77),将核酸检测阳性的77份样本全部感染VERO-E6细胞获得病毒株32株,阳性率为38.55%(32/77)。结论实时荧光PCR法更适合于新布尼亚病毒感染者早期实验室快速诊断,发病在1w左右的病例不适合用ELISA的方法做确证实验。细胞培养分离病毒虽然是金标准,但耗时、费力,对早期诊断意义不大,但适于研究或疫苗研发。
[Abstract]:Objective to detect and compare the acute phase blood of suspected febrile patients with thrombopenia syndrome in 2013 by real-time fluorescence PCR, enzyme-linked immunosorbent assay (Elisa) and cell culture, and to find a rapid and accurate method for the identification of new Bunia virus infection. Methods the blood samples of 180 suspected clinical cases in acute phase were detected by real-time fluorescence PCR, and the positive samples were screened out. At the same time, the positive samples were detected by enzyme-linked immunosorbent assay (Elisa) and the virus strains were isolated by VERO-E6 cell culture. Results the positive rate of antibody was 42.78% by real-time fluorescence PCR (77/180Ct 鈮，

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